This information is crucial to understand the effectiveness of nanosized silver sulfadiazine loaded in poloxamer thermosensitive hydrogel (NS/Gel) and its safety in vivo.Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus (MRSA) are the most common microbial strains found in patients with infected wounds. Metal nanoparticles (NPs) like zinc (Zn), silver (Ag), and gold (Au) have bacteriostatic/bactericidal activity including low in vivo toxicity and they are suggested wound dressings. 2 Previous study reported that the combination of silver sulfadiazine (AgSD) nanosuspensions and thermoresponsive hydrogel decreased cytotoxicity using the methyl thiazolyltetrazolium (MTT) assay in the L929 mouse fibroblast cell lines and improved AgSD antibacterial activity in vitro. 3 In this letter, we would to communicate our thoughts on the staining method used to examine the inflammation response in this study.In this paper, the author mentioned that transforming growth factor β (TGF-β), vascular endothelial growth factor A (VEGF-A), and interleukin 6 (IL-6) are mainly evolved during three phases of wound healing, which include the inflammatory stage, granulation tissue formation, and collagen fiber production. TGF-β can regulate wound healing and fibrosis by increasing the expression of collagen type I. VEGF-A plays a role in vasculogenesis by promoting metabolic activity for cell proliferation and collagen synthesis. Furthermore, IL-6 indicated an inflammatory response following wound induction. Lei et al reported that growth factors are secreted into injured areas to regulate cellular responses during the healing process of wounds. These responses include promoting angiogenesis, re-epithelialization, and reducing the inflammatory response. Concerning IL-6, tumor necrosis factor α (TNF-α) is another marker of inflammation. 4 Treatment for skin wounds aims to minimize the formation of scar tissue, promote quick wound healing, and restore the natural tissue structure and functioning of damaged skin. As a result, more specifics of wound repair under various circumstances were assessed in rat model studies. Skin wounds are frequently vulnerable to microbes that could cause inflammation and so inhibit the healing process. In this paper, the author reported that to measure the cytokines, they used Enzyme-Linked Immunosorbent Assay (ELISA). IL-6 expression increased on the first day, then decreased over the next few days. The increase in IL-6 expression indicated an inflammatory response following wound induction.
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