Nematicidal potential of essential oils (EOs) has been widely reported. Terpenoids present in most of the essential oils have been reported responsible for their bioactivity though very less is known about their modes of action. In the present study, an in vitro screening of nine Eos, namely, Citrus sinensis (OEO), Myrtus communis (MTEO), Eucalyptus citriodora (CEO), Melaleuca alternifolia (TEO), Acorus calamus (AEO), Commiphora myrrha (MREO), Cymbopogon nardus (CNEO), Artemisia absinthium (WEO), and Pogostemon cablin (PEO) against Meloidogyne incognita revealed OEO, CNEO, and TEO as most effective with LC50 39.37, 43.22, and 76.28 μg ml–1 respectively. EOs had varying compositions of mono- and sesquiterpenes determined by gas chromatography-mass spectrometry (GC-MS) analysis. The in silico molecular interactions screening of major EO constituents and the seven selected target proteins of the nematode indicated highest binding affinity of geraniol-ODR1 (odorant response gene 1) complex (ΔG = -36.9 kcal mol–1), due to extensive H-bonding, hydrophobic and π-alkyl interactions. The relative binding affinity followed the order: geraniol-ODR1 > β-terpineol-ODR1 > citronellal-ODR1 > l-limonene-ODR1 > γ-terpinene-ODR1. Taken together, the cumulative in vitro and computational bioefficacy analysis related to the chemoprofiles of EOs provides useful leads on harnessing the potential of EOs as bionematicides. The insight on biochemical ligand–target protein interactions described in the present work will be helpful in logical selection of biomolecules and essential oils for development of practically viable bionematicidal products.

Study ascertained the recovery of β-carotene from enzyme-treated (enzyme load of 167 U/g) pericarp of ripe bitter melon using supercritical fluid extraction (SFE) technique. Effect of different pressure (ranged from 150–450 bar), carbon dioxide (CO2) flow rates (ranged from 15 to 55 ml/min), temperatures (from 50 to 90 °C), and extraction periods (from 45–225 minutes) were observed on the extraction efficiency of β-carotene. Results showed that extraction pressure (X1) among extraction parameters had the most significant (p < 0.05) effect on extraction efficiency of the β-carotene followed by allowed extraction time (X4), CO2 flow rate (X2) and the temperature of the extraction (X3). The maximum yield of 90.12% of β-carotene from lyophilized enzymatic pretreated ripe bitter melon pericarp was achieved at the pressure of approx. 390 bar, flow rate of 35 mL/min, temperature at 70 °C and extraction time of 190 min, respectively. Based on the accelerated storage study the 70% retention shelf life of the β-carotene into extract was estimated up to 2.27 months at 10 °C and up to 3.21 months at 5 °C.

A method of construction of row-column designs for estimation of main effects and two factor interaction effects in 2 n factorial microarray experiments based on orthogonal parameterization has been developed in minimum number of replications. A catalogue of designs for 2 B n B 9 has been prepared. The catalogue also gives the main effects and two-factor interactions confounded in different replications and the factorial effects that are not confounded in a replication. The efficiency factor of estimable main effects and two-factor interactions has been given. For each 2 n factorial, two designs have been given, one in which main effects are estimated with more efficiency and another in which two-factor interactions are estimated with more efficiency. A procedure of construction of row-column designs for estimation of all factorial effects with odd number of factors has been given. Row-column designs with unequal replication of different treatment combinations have also been obtained for estimation of all main effects and two-factor interactions.

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In this study, an underutilized citrus family fruit named grapefruit was explored for the extraction of lycopene using supercritical carbon dioxide (CO2) extraction technique. An experimental design was developed using response surface methodology to investigate the effect of supercritical carbon dioxide (CO2) operating parameter viz., pressure, temperature, CO2 flow rate, and extraction time on the extraction yield of lycopene yield from grapefruit. A total of 30 sets of experiments were conducted with six central points. The statistical model indicated that extraction pressure and extraction time individually, and their interaction, significantly affected the lycopene yield. The central composite design showed that the polynomial regression models developed were in agreement with the experimental results, with R2 of 0.9885. The optimum conditions for extraction of lycopene from grapefruit were 305 bar pressure, 35 g/min CO2 flow rate, 135 min of extraction time, and 70 °C temperature.

Improving the farming system to attain the household level self‐sufficiency, land utilization efficiency and sustainable livelihood security depends on better socio‐economic and ecological aspects of the systems practiced by the small farmers in the semiarid regions of India. The higher sustainable livelihood security index (SLSI) in the integrated crops‐livestock system helps to restore economic and ecological sustainability. The aim of this study was to analyze how various modules of farming in different combinations have interacted with the diversification of existing systems. We used the ecological security index (ESI), economic efficiency index (EEI), and social equity index (SEI) to produce a SLSI of improved integrated farming system (IFS) compared with benchmark farming in semiarid regions. In this study, different existing IFS comprising of seven modules (field crops, dairy, goats, poultry, horticulture, fishery, and apiary) in different combinations were examined. Results revealed that 72.5% farmers preferred to integrate two modules, where as 95% of farmers adopted field crops + dairy (FC + D) in preference to other modules. The sustainability indicators: ESI (+43.3%), EEI (+16.0%), SEI (+11.6%), and SLSI (+6.0%) were improved in the FC + D farming system to a greater degree than other IFS module combinations. Similarly, improved IFS interventions also increased sustainability indicators over benchmark farming. Based on large scale household studies over 5 years, our findings suggest that the improved IFS succeeded in providing a wider array of livelihood security than existing practices. Hence, the paradigm shift from component approach to an IFS‐based resilient system, which is economically viable, environmentally sustainable, and socially acceptable, is needed for development of semiarid regions.

Environmental concerns related to synthetic pesticides and the emphasis on the adoption of an integrated pest management concept as a cardinal principle have strengthened the focus of global research and development on botanical pesticides. A scientific understanding of the mode of action of biomolecules over a range of pests is key to the successful development of biopesticides. The present investigation focuses on the in silico protein-ligand interactions of allyl isothiocyanate (AITC), a major constituent of black mustard (Brassica nigra) essential oil (MEO) against two pests, namely, Meloidogyne incognita (Mi) and Fusarium oxysporum f. sp. lycopersici (Fol), that cause severe yield losses in agricultural crops, especially in vegetables. The in vitro bioassay results of MEO against Mi exhibited an exposure time dependent on the lethal concentration causing 50% mortality (LC50) values of 47.7, 30.3, and 20.4 μg ml−1 at 24, 48, and 72 h of exposure, respectively. The study revealed short-term nematostatic activity at lower concentrations, with nematicidal activity at higher concentrations upon prolonged exposure. Black mustard essential oil displayed excellent in vitro Fol mycelial growth inhibition, with an effective concentration to cause 50% inhibition (EC50) value of 6.42 μg ml−1. In order to decipher the mechanism of action of MEO, its major component, AITC (87.6%), which was identified by gas chromatography–mass spectrometry (GC-MS), was subjected to in silico docking and simulation studies against seven and eight putative target proteins of Mi and Fol, respectively. Allyl isothiocyanate exhibited the highest binding affinity with the binding sites of acetyl cholinesterase (AChE), followed by odorant response gene-1 (ODR1) and neuropeptide G-protein coupled receptor (nGPCR) in Mi, suggesting the possible suppression of neurotransmission and chemosensing functions. Among the target proteins of Fol, AITC was the most effective protein in blocking chitin synthase (CS), followed by 2,3-dihydroxy benzoic acid decarboxylase (6m53) and trypsinase (1try), thus inferring these as the principal molecular targets of fungal growth. Taken together, the study establishes the potential of MEO as a novel biopesticide lead, which will be utilized further to manage the Mi–Fol disease complex.

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