Plants are sessile organisms, and their ability to adapt to stress is crucial for survival in natural environments. Many observations suggest a relationship between stress tolerance and heat shock proteins (HSPs) in plants, but the roles of individual HSPs are poorly characterized. We report that transgenic Arabidopsis plants expressing less than usual amounts of HSP101, a result of either antisense inhibition or cosuppression, grew at normal rates but had a severely diminished capacity to acquire heat tolerance after mild conditioning pretreatments. The naturally high tolerance of germinating seeds, which express HSP101 as a result of developmental regulation, was also profoundly decreased. Conversely, plants constitutively expressing HSP101 tolerated sudden shifts to extreme temperatures better than did vector controls. We conclude that HSP101 plays a pivotal role in heat tolerance in Arabidopsis. Given the high evolutionary conservation of this protein and the fact that altering HSP101 expression had no detrimental effects on normal growth or development, one should be able to manipulate the stress tolerance of other plants by altering the expression of this protein. INTRODUCTIONOrganisms have evolved a wide array of mechanisms for adapting to stressful environments. One of the most closely studied of these is the induction of heat shock proteins (HSPs), which comprise several evolutionarily conserved protein families. All of the major HSPs (that is, those expressed in very high amounts in response to heat and other stresses) have related functions: they ameliorate problems caused by protein misfolding and aggregation. However, each major HSP family has a unique mechanism of action. Some promote the degradation of misfolded proteins (Lon, ubiquitin, and various ubiquitin-conjugating enzymes); others bind to different types of folding intermediates and prevent them from aggregating (Hsp70 and Hsp60); and still another (Hsp100) promotes the reactivation of proteins that have already aggregated Lindquist, 1993, 1994).Although all organisms synthesize HSPs in response to heat, the balance of proteins synthesized and the relative importance of individual HSP families in stress tolerance vary greatly among organisms. For example, in yeast , a member of the Hsp100 (ClpB/C) family, Hsp104, is strongly expressed in the nuclear-cytoplasmic compartment in response to stress and plays a particularly pivotal role in tolerance to extreme conditions (Sanchez et al., 1992;. Yeast cells expressing Hsp104 survive exposure to high temperatures or high concentrations of ethanol 1000-to 10,000-fold better than do cells not expressing Hsp104. Members of the Hsp100 family also play critical roles in the stress tolerance of bacterial cells (Schirmer et al., 1996), including photosynthetic cyanobacteria (Eriksson and Clarke, 1996). In contrast, the fruit fly Drosophila makes no protein of this type in response to stress; instead, the induction of Hsp70 plays the central role in stress tolerance in this organism (Solomon et al., 19...
Leaf senescence, which constitutes the final stage of leaf development, involves programmed cell death and is intricately regulated by various internal and environmental signals that are incorporated with age-related information. ABA plays diverse and important physiological roles in plants, and is involved in various developmental events and stress responses. ABA has long been regarded as a positive regulator of leaf senescence. However, the cellular mediators of ABA-induced senescence have not been identified. We sought to understand the ABA-induced senescence signaling process in Arabidopsis by examining the function of an ABA- and age-induced gene, RPK1, which encodes a membrane-bound, leucine-rich repeat-containing receptor kinase (receptor protein kinase 1). Loss-of-function mutants in RPK1 were significantly delayed in age-dependent senescence. Furthermore, rpk1 mutants exhibited reduced sensitivity to ABA-induced senescence but little change to jasmonic acid- or ethylene-induced senescence. RPK1 thus mediates ABA-induced leaf senescence as well as age-induced leaf senescence. Conditional overexpression of RPK1 at the mature stage clearly accelerated senescence and cell death, whereas induction of RPK1 at an early developmental stage retarded growth without triggering senescence symptoms. Therefore, RPK1 plays different roles at different stages of development. Consistently, exogenously applied ABA affected leaf senescence in old leaves but not in young leaves. The results, together, showed that membrane-bound RPK1 functions in ABA-dependent leaf senescence. Furthermore, the effect of ABA and ABA-inducible RPK1 on leaf senescence is dependent on the age of the plant, which in part explains the mechanism of functional diversification of ABA action.
In response to environmental challenges, plant cells activate several signaling pathways that trigger the expression of transcription factors. Arabidopsis MYB60 was reported to be involved in stomatal regulation under drought conditions. Here, two splice variants of the MYB60 gene are shown to play a crucial role in stomatal movement. This role was demonstrated by over-expressing each variant, resulting in enhanced sensitivity to water deficit stress. The MYB60 splice variants, despite the fact that one of which lacks the first two exons encoding the first MYB DNA binding domain, both localize to the nucleus and promote guard cell deflation in response to water deficit. Moreover, MYB60 expression is increased in response to a low level of ABA and decreased in response to high level of ABA. At initial stage of drought stress, the plant system may modulate the root growth behavior by regulating MYB60 expression, thus promotes root growth for increased water uptake. In contrast, severe drought stress inhibits the expression of the MYB60 gene, resulting in stomatal closure and root growth inhibition. Taken together, these data indicate that MYB60 plays a dual role in abiotic stress responses in Arabidopsis through its involvement in stomatal regulation and root growth.
Glioblastoma (GBM) is the most aggressive, neurologically destructive and deadly tumor of the central nervous system (CNS). In GBM, the transcription factors NF-κB and STAT3 are aberrantly activated and associated with tumor cell proliferation, survival, invasion and chemoresistance. In addition, common activators of NF-κB and STAT3, including TNF-α and IL-6, respectively, are abundantly expressed in GBM tumors. Herein, we sought to elucidate the signaling crosstalk that occurs between the NF-κB and STAT3 pathways in GBM tumors. Using cultured GBM cell lines as well as primary human GBM xenografts, we elucidated the signaling crosstalk between the NF-κB and STAT3 pathways utilizing approaches that either a) reduce NF-κB p65 expression, b) inhibit NF-κB activation, c) interfere with IL-6 signaling, or d) inhibit STAT3 activation. Using the clinically relevant human GBM xenograft model, we assessed the efficacy of inhibiting NF-κB and/or STAT3 alone or in combination in mice bearing intracranial xenograft tumors in vivo. We demonstrate that TNF-α-induced activation of NF-κB is sufficient to induce IL-6 expression, activate STAT3, and elevate STAT3 target gene expression in GBM cell lines and human GBM xenografts in vitro. Moreover, the combined inhibition of NF-κB and STAT3 signaling significantly increases survival of mice bearing intracranial tumors. We propose that in GBM, the activation of NF-κB ensures subsequent STAT3 activation through the expression of IL-6. These data verify that pharmacological interventions to effectively inhibit the activity of both NF-κB and STAT3 transcription factors must be used in order to reduce glioma size and aggressiveness.
SummaryThe Arabidopsis hot2 mutant was originally identified based on its lack of thermotolerance, but pleiotropic abnormal phenotypes are also exhibited under normal conditions, including semi-dwarfism, ethylene overproduction and aberrant cell shape with incomplete cell walls. Here we present additional characterization of the hot2 mutant, and the map-based cloning of HOT2. Mutants of hot2 had an aberrant tolerance to salt and drought stresses, and accumulated high levels of Na þ in cells under either normal or NaCl stress conditions. Expression of the stress-inducible COR15A and KIN1 gene in hot2 mutants in response to increased NaCl concentrations was normal. HOT2 encoded a chitinase-like protein (AtCTL1) that has not previously been shown to be involved in tolerance to salt stress. Ten-day-old seedlings of wild-type plants exhibited constitutive expression of the AtCTL1 transcript, the level of which was unaffected by treatment with NaCl, mannitol or mild heat. These observations provide genetic evidence that a chitinase-like protein prevents the overaccumulation of Na þ ions, thereby contributing to the salt tolerance in Arabidopsis. A possible role for this chitinase-like protein in Arabidopsis tolerance to abiotic stress is discussed.
To evaluate the genetic control of stress responses in Arabidopsis, we have analyzed a mutant (uvh6-1) that exhibits increased sensitivity to UV light, a yellow-green leaf coloration, and mild growth defects. We have mapped the uvh6-1 locus to chromosome I and have identified a candidate gene, AtXPD, within the corresponding region. This gene shows sequence similarity to the human (Homo sapiens) XPD and yeast (Saccharomyces cerevisiae) RAD3 genes required for nucleotide excision repair. We propose that UVH6 is equivalent to AtXPD because uvh6-1 mutants carry a mutation in a conserved residue of AtXPD and because transformation of uvh6-1 mutants with wild-type AtXPD DNA suppresses both UV sensitivity and other defective phenotypes. Furthermore, the UVH6/AtXPD protein appears to play a role in repair of UV photoproducts because the uvh6-1 mutant exhibits a moderate defect in the excision of UV photoproducts. This defect is also suppressed by transformation with UVH6/AtXPD DNA. We have further identified a T-DNA insertion in the UVH6/AtXPD gene (uvh6-2). Plants carrying homozygous insertions were not detected in analyses of progeny from plants heterozygous for the insertion. Thus, homozygous insertions appear to be lethal. We conclude that the UVH6/AtXPD gene is required for UV resistance and is an essential gene in Arabidopsis.DNA damage is a challenge for all organisms exposed to UV irradiation. UV photoproducts consist primarily of cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidinone dimers (Mitchell and Nairn, 1989;Pfeifer, 1997). These lesions inhibit DNA replication and transcription and also promote mutagenesis (McGregor, 1999). The effects of UV irradiation are especially detrimental in plants, where sunlight is both a source of damage and a requirement for photosynthesis.Increasing evidence suggests that plants repair UVdamaged chromosomes using mechanisms similar to those found in humans (Homo sapiens) and yeast (Saccharomyces cerevisiae). These mechanisms include the nucleotide excision repair (NER) pathway, a process which involves recognition of UV lesions, incision of the damaged strand on both sides of the lesion, removal of the damaged fragment, and repair by gap filling and ligation (Batty and Wood, 2000;de Boer and Hoeijmakers, 2000;Prakash and Prakash, 2000). Several potential plant homologs of human and yeast NER genes have been identified. Genetic analyses of these plant genes, including studies of the phenotypes of plants carrying mutations within these genes, provide support for the idea that the NER pathway is conserved in plants.Lesion recognition during NER involves the homologous heterodimers XPC:HR23B (human) and RAD4:RAD23 (yeast; Balajee and Bohr, 2000;Batty and Wood, 2000;de Boer and Hoeijmakers, 2000;Prakash and Prakash, 2000). The Arabidopsis genome contains potential homologs of both XPC/RAD4 and HR23B/RAD23 (Arabidopsis Genome Initiative, 2000). HR23B expression occurs in Arabidopsis, rice (Oryza sativa), and carrot (Daucus carota), and the carrot gene complements the r...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.