Aqueous extraction of coconut oil with various enzymes was investigated. Several enzyme preparations (cellulase, polygalacturonase, protease, and α‐amylase) were used at different concentrations, pH, and temperature values to enhance oil extraction. After the oil had been released by the enzyme reaction, it was separated by centrifugation. The results showed that an enzyme mixture at 1% (w/w) each of cellulase, α‐amylase, polygalacturonase, and protease at pH 7.0 and an extraction temperature of 60°C represented the most effective extraction conditions with an oil yield of 73.8%. Quality characteristics of the oil were as follows: moisture content, 0.11%; free fatty acid, 0.051%; peroxide value, 0.016 meq oxygen/kg; anisidine value, 0.026; iodine value, 8.3; saponification value, 260; and color, 0.6 (Y+5R). This technique for recovering oil from fresh coconut meat with enzymes is a significant improvement in both oil yield and quality over the traditional wet process.
Three methods for coconut oil extration using acetic acid, baker's yeast, and mixed enzymes were investigated. Coconut milk was allowed to settle for two hours; for cream separation. When the cream reacted with 25 % acetic acid at 0.l % ‑ 0.4 % levels or baker's yeast at 0.5 ‑ 2 g levels for 10 ‑ 14 hours, the oil was separated into two phases; the upper phase containing coconut oil‑rich fraction and the lower phase consisting of water. The oil phase was finally boiled for 20 minutes to remove moisture. The other extraction method was based on the combined action of cellulase, a ‑amylase, protease, and poly‑galacturonase at 0.1 % to 1 % on grated coconut meat at pH 4 to 8, 400C to 600C for 30 minutes. Oil recovely, moisture content, FFA, peroxide value, saponification value, anisidine value, iodine value and colour of the oil were studied. Up to 60 % recovery of high quality oil was obtained by acetic acid or baker's yeast treatment whilst that of mixed enzymes treatment was 73 %. These three alternatives wet processing showed significant improvement as compared to the traditional process.
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