This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different meatball shops were isolated and amplified, and then the PCR amplicon was digested by BseDI restriction enzyme to detect the presence of pork in meatballs. BseDI restriction enzyme was able to cleave porcine cytochrome b gene into two fragments (131 bp and 228 bp). Testing the meatballs from the local market showed that nine of twenty meatball shops in Yogyakarta region were detected to have pork contamination, but there was no pork contamination in meatball shops in Surabaya region. In conclusion, specific PCR amplification of cytochrome b gen and cleaved by BseDI restriction enzymes seems to be a powerful technique for the identification of pork presence in meatball because of its simplicity, specificity and sensitivity. Furthermore, pork contamination intended for commercial products of sausage, nugget, steak and meat burger can be checked. The procedure is also much cheaper than other methods based on PCR, immunodiffusion and other techniques that need expensive equipment.
Penelitian ini bertujuan untuk menganalisis kemampuan representasi visual mahasiswa program studi Pendidikan Guru Sekolah Dasar dalam menyajikan data pada mata kuliah Statistik Pendidikan. Penelitian ini merupakan penelitian deskriptif kualitatif dengan subjek penelitian adalah mahasiswa program studi Pendidikan Guru Sekolah Dasar STKIP PGRI Pacitan. Data dikumpulkan dari hasil jawaban mahasiswa dalam membuat tabel, diagram lingkaran, dan diagram batang. Analisis data menggunakan model analisis interaktif yang meliputi pengumpula data, reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa kemampuan representasi visual mahasiswa dalam membuat tabel distribusi frekuensi baik dengan menggunakan aplikasi komputer maupun menggambar manual memenuhi indikator representasi visual. Dalam mengilustrasikan diagram lingkaran dengan menggunakan aplikasi komputer memenuhi indikator representasi visual, sedangkan mahasiswa yang menggambar manual tidak memenuhi indikator. Dan dalam mengilustrasikan diagram batang menggunakan aplikasi komputer tidak memenuhi indikator, sedangkan mahasiswa yang menggambar manual memenuhi indikator representasi visual.
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