One of the distinguishing features of the gonadotropin and thyrotropin hormone family is their heterodimeric structure, consisting of a common a subunit and a hormone-specific f8 subunit. Subunit assembly is vital to the function of these hormones: The conformation of the heterodimer is essential for controlling secretion, hormonespecific posttranslational modifications, and signal transduction. To address whether a and ,3 subunits can be synthesized as one chain and also maintain biological activity, a chimera composed of the human chorionic gonadotropin (hCG) ,3 subunit genetically fused to the a subunit was constructed. question of whether the common a subunit and a hormonespecific (3 subunit can be synthesized as a single chain to achieve the biological response. For structure-function studies, the ability to express a heterodimer as a single biologically active chain would likely avoid mutagenesis-induced defects in combination and secretion of individual subunits. In addition, since subunit dissociation will inactivate the in vivo activity of the heterodimer, single-chain analogs could have a longer biological half-life. A chimera composed of the C-terminal end of hCG(3 genetically fused to the N-terminal end of the a subunit was constructed because these regions can be modified without significant effects on the biological activity of the heterodimer (7,8). Expression of this chimeric gene in CHO cells produced a single polypeptide hCG molecule that was biologically active in vitro and in vivo. These results demonstrate that the a and hCG,B subunits encoded as single chain can fold into an appropriate conformation and that a noncovalent linkage of the a and 13 subunits is not required for biological activity. This approach can be used to further investigate structure-function relationships of hCG and related glycoprotein hormones that were previously not tractable because of the absolute dependence on subunit assembly for biological activity.
The gonadotropin/thyrotropin hormone family is characterized by a heterodimeric structure composed of a common ␣ subunit noncovalently linked to a hormonespecific  subunit. The conformation of the heterodimer is essential for controlling secretion, hormone-specific post-translational modifications, and signal transduction. Structure-function studies of follicle-stimulating hormone (FSH) and the other glycoprotein hormones are often hampered by mutagenesis-induced defects in subunit combination. Thus, the ability to overcome the limitation of subunit assembly would expand the range of structure-activity relationships that can be performed on these hormones. Here we converted the FSH heterodimer to a single chain by genetically fusing the carboxyl end of the FSH  subunit to the amino end of the ␣ subunit in the presence or absence of a linker sequence. In the absence of the CTP linker, the secretion rate was decreased over 3-fold. Unexpectedly, however, receptor binding/signal transduction was unaffected by the absence of the linker. These data show that the single-chain FSH was secreted efficiently and is biologically active and that the conformation determinants required for secretion and biologic activity are not the same.One of the hallmarks of the gonadotropin and thyrotropin hormone family is their heterodimeric structure, consisting of a common ␣ subunit and a unique  subunit (1). Subunit assembly is vital to the function of these hormones: (i) only the dimers are bioactive, (ii) maturation of the hormone-specific oligosaccharides is dependent on the formation of the heterodimer complex, and (iii) the secretion efficiency of the dimer is determined by the  subunit. Previously, we constructed a chimera composed of the human chorionic gonadotropin (hCG) 1  subunit genetically fused to the ␣ subunit, and the resulting single polypeptide chain was efficiently secreted and was biologically active (2). Because subunit dissociation would lead to inactivation of the heterodimer, a single-chain form could have higher biological activity.Although the gonadotropin dimers have similar structural features, they are nevertheless unique. The ␣ subunit in the dimers has a different conformation which is manifested by distinct immunological and spectral characteristics (3-6). In addition, the carbohydrates on the ␣ subunits are not the same in all the gonadotropin dimers (7-9) and the receptor contact sites on the ␣ subunit differ among the hormones (10, 39, 40). Thus, a priori one cannot predict based on the CG model that other members of the glycoprotein hormone family can be converted to single chains.Tethering a variety of multisubunit complexes into single chains has been performed by several laboratories to increase protein stability or activity (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). In such studies, a linker sequence was designed to give optimal alignment of determinants. In the case of the glycoprotein hormones, we presumed that a linker would be required for successful expression of the correspondin...
The hCG beta-subunit contains a carboxy-terminal extension bearing four serine-linked oligosaccharides [carboxy-terminal peptide (CTP)], which is important for maintaining its longer half-life compared with the other glycoprotein hormones. Previously, we enhanced the in vivo half-life of FSH by fusing the CTP to the carboxy end of FSH beta coding sequence. The alpha-subunit is common to the glycoprotein family. We constructed alpha-subunit CTP chimeras, since such analogs with the appropriate O-linked glycosylation and conformation would increase the in vivo stability of the entire glycoprotein hormone family. Two chimeras were constructed using overlapping polymerase chain reaction mutagenesis: a variant with CTP at the carboxy end and another analog with the CTP at the N-terminal region of the subunit, between amino acids 3 and 4. The latter design was based on models showing that the amino-terminal region of alpha is not involved in assembly with the beta-subunit, nor is it essential for receptor binding and signal transduction. These chimeras were cotransfected with the hCG beta gene into Chinese hamster ovary cells. The chimeras were secreted and combined efficiently with the CG beta-subunit, comparable to the wild type alpha-subunit. CG dimers containing the alpha-subunit chimera with CTP at the carboxy end of the subunit had a much lower binding affinity for the hLH-hCG receptor in vitro, whereas the binding of the dimer containing the CTP at the amino-terminal end of the subunit was similar to wild type hCG. Furthermore, the in vivo activity of this analog was enhanced significantly. Moreover, regardless of the two insertion points in the alpha-subunit, the CTP sequence was O-glycosylated. These data suggest that the entire signal for O-glycosylation is primarily contained within the CTP sequence and is not dependent on the flanking regions of the recipient protein. The transfer of CTP to the alpha-subunit of hCG results in an agonist with prolonged biological action in vivo. These data further support the rationale for using the CTP as a general target to increase the potency of bioactive glycoproteins.
One of the major structural differences between the LH beta and CG beta subunits is the carboxy-terminal region: beyond amino acid 114, LH beta has a hydrophobic heptapeptide stretch, while CG beta contains a 31-amino acid hydrophilic carboxy-terminal peptide (CTP) that is O-glycosylated. The CG beta subunit is secreted quantitatively as a monomer and assembles efficiently whereas secretion and assembly of LH beta is inefficient. We previously implicated the carboxy-terminal heptapeptide as a determinant for the different intracellular behavior manifested by the LH beta subunit compared with the CG beta subunit. Here we tested the function of the heptapeptide and CTP domains by fusing them to their counterparts at amino acid 114 of CG beta or LH beta subunits. The secretion and assembly of these chimeras were examined in transfected Chinese hamster ovary cells. Removal of the heptapeptide enhanced the amount of LH beta subunit secreted 4-fold compared with intact LH beta. Fusion of this heptapeptide to CG beta 114, i.e. CG beta lacking the CTP, decreased the amount of secreted subunit 2-fold compared with wild type human CG beta. Similar experiments reveal that although deleting the CTP from the CG beta subunit did not significantly alter secretion, the combination efficiency of the truncated subunit was reduced to 60%. Perturbing the native carboxy-terminal sequence of either subunit increased the heterogeneity of the secreted forms. This result suggests that these regions are also involved in the posttranslational processing of the asparagine-linked oligosaccharides of the beta-subunits. Fusion of the LH beta heptapeptide to the truncated CG beta subunit decreased combination with the alpha-subunit. These data further support the hypothesis that the carboxy-terminal regions of LH beta and CG beta subunits play a role in the intracellular behavior of the corresponding heterodimers.
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