Curcumin exhibits potent anticancer
activity via various mechanisms,
but its in vivo efficacy has been hampered by poor solubility. Nanotechnology
has been employed to deliver curcumin, but most of the reported systems
suffered from low drug loading capacity and poor stability. Here,
we report the development and optimization of a liposomal formulation
for curcumin (Lipo-Cur) using an automated microfluidic technology.
Lipo-Cur exhibited a mean diameter of 120 nm with a low polydispersity
index (<0.2) and superior loading capacity (17 wt %) compared to
other reported liposomal systems. Lipo-Cur increased the water solubility
of curcumin by 700-fold, leading to 8–20-fold increased systemic
exposure compared to the standard curcumin suspension formulation.
When coadministered with cisplatin to tumor-bearing mice, Lipo-Cur
augmented the antitumor efficacy of cisplatin in multiple mouse tumor
models and decreased the nephrotoxicity. This is the first report
demonstrating the dual effects of curcumin enabled by a nanoformulation
in enhancing the efficacy and reducing the toxicity of a chemo-drug
in animal models under a single and low dose administration.
Colorectal cancer with peritoneal metastases is currently treated by cytoreductive surgery and locoregional chemotherapeutics. This standard treatment is associated with high morbidity, mortality, and recurrence rate. To augment the existing therapy, we developed a liposome-based delivery system containing 1,2-stearoyl-3-trimethylammonium-propane chloride (DSTAP), a cationic lipid, to localize a toll-like receptor agonist, resiquimod (R848), in the peritoneal cavity (PerC) for enhancing the immune response against cancer that had spread to the PerC. The liposomes delivered by intraperitoneal injection increased peritoneal retention of R848 by 14-fold while retarding its systemic absorption, leading to a 5-fold decreased peak plasma concentration compared to free R848 in mice. Within the PerC, the DSTAP-liposomes were found in ~40% of the dendritic cells by flow cytometry. DSTAP-R848 significantly upregulated interferon α (IFN-α) in the peritoneal fluid by 2-fold compared to free R848, without increasing the systemic level. Combined with oxaliplatin, a cytotoxic agent inducing immunogenic cell death, DSTAP-R848 effectively inhibited the progression of CT26 murine colorectal tumor in the PerC, while the combination with free R848 only showed a mild effect. Moreover, the combination of oxaliplatin and DSTAP-R848 significantly increased infiltration of CD8+ T cells in the PerC compared to oxaliplatin combined with free R848, indicating enhanced immune response against the tumor. The results suggest that DSTAP-R848 exhibits potential in augmenting existing therapies for treating colorectal cancer with peritoneal metastases via immune activation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.