The objective of this study was the development of a bioactive glass coating on zirconia (Zr) to modulate the gingival fibroblast phenotype. For this purpose, Biosilicate® (BS) particles in a water/isopropyl alcohol (1:1) vehicle (6 mg/mL) were applied to zirconia discs followed by thermal treatment at 1100 °C for 20 min. The surface topography (SEM), chemical composition (EDX), surface roughness (Ra; confocal microscopy), surface free energy (goniometry), and color alteration (UV-vis spectrophotometry) were assessed (n=6). Thereafter, L929 fibroblasts were seeded onto Zr and Zr+BS discs, and cell proliferation (Alamar Blue; n=6), morphology (SEM; n=2), migration (wound healing; n=4), and collagen synthesis (Sirius Red; n=6) were evaluated up to 7 days. Data were analyzed by ANOVA/Tukey tests (a=5%). A homogeneous coating consisting of Si, Na, O, and Ca was detected on the Zr surface after thermal treatment with BS, which led to a significant increase in surface roughness and free energy (p<0.05). No change in color parameters was observed (p>0.05). Cells seeded on the Zr+BS surface featured increased proliferation, collagen expression, and migration capability in comparison with those cultured on plain Zr (p<0.05). SEM images revealed that cell spreading occurred faster in the presence of BS. Therefore, it was concluded that thermal treatment of the Zr surface with BS led to the deposition of a bioactive coating, which induced gingival fibroblast spread, proliferation, migration, and collagen expression in vitro.
Objetivo: avaliar a precisão dimensional de modelos de gesso tipos III e IV submetidos a diversos tipos de fraturas, seguidas de colagem com cianoacrilato. Materiais e mé-todo: a amostra foi constituída por 80 modelos de gesso IntroduçãoOs modelos de gesso utilizados na prática odontológica podem ser acometidos por fraturas durante a sua manipulação em consultório e laboratório de prótese. As fraturas dos modelos de gesso utilizados para confecção de próteses fixas ocorrem, com mais frequência, durante a remoção do modelo, especialmente quando o material de moldagem oferece resistência e/ou quando os preparos dentários são longos e finos 1 . Diante da necessidade de colagem dos fragmentos, estudo anterior avaliou os efeitos desse procedimento e os autores sugeriram que é possível realizá-lo na prática laboratorial, sem afetar a precisão final da prótese 2 .O adesivo de cianoacrilato tem sido utilizado para colar fragmentos de gesso com resultados satisfatórios 3 . Também tem sido usado para melhorar as propriedades de modelos de gesso através da aplicação de uma fina camada sobre o gesso, aumentando assim, a resistência à fratura e abrasão 4,5 . Reduz ainda a absorção de água 6 e quando aplicado no troquel de gesso preserva a margem do preparo 4,6 . Além disso, os adesivos à base de cianoacrilato são simples de usar, polimerizam ao reagir
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