The vertebrate lens provides an excellent model with which to study the mechanisms required for epithelial invagination. In the mouse, the lens forms from the head surface ectoderm. A domain of ectoderm first thickens to form the lens placode and then invaginates to form the lens pit. The epithelium of the lens placode remains in close apposition to the epithelium of the presumptive retina as these structures undergo a coordinated invagination. Here, we show that F-actin-rich basal filopodia that link adjacent presumptive lens and retinal epithelia function as physical tethers that coordinate invagination. The filopodia, most of which originate in the presumptive lens, form at E9.5 when presumptive lens and retinal epithelia first come into close contact, and have retracted by E11.5 when invagination is complete. At E10.5 -the lens pit stage -there is approximately one filopodium per epithelial cell. Formation of filopodia is dependent on the Rho family GTPase Cdc42 and the Cdc42 effector IRSp53 (Baiap2). Loss of filopodia results in reduced lens pit invagination. Pharmacological manipulation of the actin-myosin contraction pathway showed that the filopodia can respond rapidly in length to change inter-epithelial distance. These data suggest that the lens-retina interepithelial filopodia are a fine-tuning mechanism to assist in lens pit invagination by transmitting the forces between presumptive lens and retina. Although invagination of the archenteron in sea urchins and dorsal closure in Drosophila are known to be partly dependent on filopodia, this mechanism of morphogenesis has not previously been identified in vertebrates.
How cone synapses encode light intensity determines the precision of information transmission at the first synapse on the visual pathway. Although it is known that cone photoreceptors hyperpolarize to light over 4-5 log units of intensity, the relationship between light intensity and transmitter release at the cone synapse has not been determined. Here, we use two-photon microscopy to visualize release of the synaptic vesicle dye FM1-43 from cone terminals in the intact lizard retina, in response to different stimulus light intensities. We then employ electron microscopy to translate these measurements into vesicle release rates. We find that from darkness to bright light, release decreases from 49 to approximately 2 vesicles per 200 ms; therefore, cones compress their 10,000-fold operating range for phototransduction into a 25-fold range for synaptic vesicle release. Tonic release encodes ten distinguishable intensity levels, skewed to most finely represent bright light, assuming release obeys Poisson statistics.
Rod and cone photoreceptors use specialized biochemistry to generate light responses that differ in their sensitivity and kinetics. However, it is unclear whether there are also synaptic differences that affect the transmission of visual information. Here, we report that in the dark, rods tonically release synaptic vesicles at a much slower rate than cones, as measured by the release of the fluorescent vesicle indicator FM1-43. To determine whether slower release results from a lower Ca 2ϩ sensitivity or a lower dark concentration of Ca 2ϩ , we imaged fluorescent indicators of synaptic vesicle cycling and intraterminal Ca 2ϩ . We report that the Ca 2ϩ sensitivity of release is indistinguishable in rods and cones, consistent with their possessing similar release machinery. However, the dark intraterminal Ca 2ϩ concentration is lower in rods than in cones, as determined by two-photon Ca 2ϩ imaging. The lower level of dark Ca 2ϩ ensures that rods encode intensity with a slower vesicle release rate that is better matched to the lower information content of dim light.
PURPOSE. The lens is a powerful model system to study integrinmediated cell-matrix interaction in an in vivo context, as it is surrounded by a true basement membrane, the lens capsule. To characterize better the function of integrin-linked kinase (ILK), we examined the phenotypic consequences of its deletion in the developing mouse lens.METHODS. ILK was deleted from the embryonic lens either at the time of placode invagination using the Le-Cre line or after initial lens formation using the Nestin-Cre line.RESULTS. Early deletion of ILK leads to defects in extracellular matrix deposition that result in lens capsule rupture at the lens vesicle stage (E13.5). If ILK was deleted at a later time-point after initial establishment of the lens capsule, rupture was prevented. Instead, ILK deletion resulted in secondary fiber migration defects and, most notably, in cell death of the anterior epithelium (E18.5 -P0). Remarkably, dying cells did not stain positively for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) or activated-caspase 3, suggesting that they were dying from a non-apoptotic mechanism. Moreover, cross to a Bax fl/fl /Bak -/-mouse line that is resistant to most forms of apoptosis failed to promote cell survival in the ILKdeleted lens epithelium. Electron microscopy revealed the presence of numerous membranous vacuoles containing degrading cellular material.CONCLUSIONS. Our study reveals a role for ILK in extracellular matrix organization, fiber migration, and cell survival. Furthermore, to our knowledge we show for the first time that ILK disruption results in non-apoptotic cell death in vivo. (Invest Ophthalmol Vis Sci. 2012;53:3067-3081)
Here, we illustrate an optical method for directly measuring the light-regulated synaptic output of neurons in the retina. The method allows simultaneous recording from many retinal neurons in intact flat-mount preparations of the vertebrate retina. These recordings depend on the use of FM1-43, an activity-dependent fluorescent dye that selectively labels synaptic vesicles. Release of the dye, which occurs upon vesicle exocytosis, is detected with 2-photon microscopy. This utilizes an infrared laser to trigger fluorescence excitation of the dye, while minimally perturbing retinal activity by activating phototransduction in rods and cones. Using this approach, one can measure activity of single neurons in the intact retinal network and populations of neurons in different layers of the retina, providing a new way to examine the function of retinal synapses and how visual information is processed.
Epithelial invagination is a central feature of embryonic morphogenesis in animals. Here, we show that RhoA mutant lens placode cells are both longer and less apically constricted than control cells, causing reduced epithelial curvature and reduced invagination. By contrast, Rac1 mutant lens placode cells are shorter and more apically restricted than controls, resulting in increased epithelial curvature and precocious closure of the lens vesicle. Quantification of RhoA and Rac1‐dependent pathway markers over the apical‐basal axis of lens pit cells showed that in RhoA mutant epithelial cells, there was a Rac1 pathway gain‐of‐function and vice versa. These findings suggest that mutual antagonism produces balanced activities of RhoA‐generated apical constriction and Rac1‐dependent cell elongation that controls cell shape and thus the curvature of invaginating epithelium. Previous studies have shown that contractile lens‐specific filopodia, under Cdc42 control, tether to the optic cup during lens placode invagination. Here, we examine the efficacy of lens filopodia in transmitting forces to co‐ordinate lens and retinal shape. Our observations suggest contractile and protrusive forces exerted by lens filopodia aid in shaping the underlying retina, and therefore bring us closer to understanding the important process of tissue co‐ordination for organ functionality.
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