Simultaneous quantification of Lopinavir and Ritonavir in tablet by HPTLC method was developed and validated. The chromatograms were developed using a mobile phase of Chloroform: 1, 4 -Dioxane (7:3 %v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 210 nm. The R f value for lopinavir and ritonavir was 0.74 and 0.58 respectively. The linearity of the method was found to be within the concentration range of 160-960 ng/spot for Lopinavir and for Ritonavir, it was 40-240 ng/spot. The lower limits of detection and quantification were 9.56 ng/spot and 28.96 ng/spot for Lopinavir and 6.82 ng/spot and 20.66 ng/spot for Ritonavir. The method was also validated for precision, specificity and recovery. This developed method was used to analyze fixed-dose tablet (Lopimune, Cipla Ltd) sample of Lopinavir and Ritonavir.
Objective: The major approach take into consideration is to develop a simple, accurate, precise and reproducible method for development and validation of UV-visible spectrophotometric method for estimation of Clopidogrel Bisulphate and Irbesartan in synthetic mixture. Methods: In linearity spectra of the Clopidogrel Bisulphate and Irbesartan to shows to possible a simultaneous equation method but the zero order linearity spectra was converted to first derivative and second derivative spectroscopic method to not shows any zero crossing point so that this method was not possible. Results: In this spectroscopic method, for Clopidogrel Bisulphate 220 nm and 250 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 10-50 μg/ml at their respective λmax with correlation coefficient (r 2) of 0.9996 and 0.9998 for Clopidogrel Bisulphate and Irbesartan, respectively. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (<2%). The limit of determination was 0.056 μg/ml and 0.075 μg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. The limit of quantification was 0.172 μg/ml and 0.229 μg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. Recovery of Clopidogrel Bisulphate and Irbesartan were found to be 99.58% and 99.66% respectively confirming the accuracy of the proposed method. % Assay was found to be 99.41% and 99.22% for Clopidogrel Bisulphate and Irbesartan, respectively. Conclusions: It can be concluded from the study that assay results obtained by proposed method are in fair agreement and can be effectively applied for the estimation of these two drugs.
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