Objective: The objective of this study was to extract the phytochemicals from leaf and stem bark of Solanum pubescens, perform qualitative analysis, and evaluate antimicrobial activity of the phytochemicals against certain microbial pathogens. Methods: Leaf and stem bark of S. pubescens was subjected to phytochemical extractions using Soxhlet apparatus with five different solvents, identified the major constituents in different solvent extracts using standard protocols and investigated the potential antimicrobial activities of the extracts against certain selected bacterial and fungal pathogens by agar well diffusion method. Results: The maximum yield was in methanolic extracts of leaf and stem bark of the study plant such as 18.51 and 12.5%, respectively, followed by the hydroalcohol extracts (14.23 and 10.00%). Qualitative phytochemical analysis revealed maximum number of extracted bioactive compounds when compared to other similar studies. Among the five different solvent extracts of S. pubescens, n-hexane extract of stem bark was found to be active against all the bacterial pathogens and stem bark extracts made with methanol and hydroalcohol showed antifungal activity against all the four fungal pathogens tested. Leaf extracts made with solvents such as n-hexane and ethyl acetate showed inhibition against Klebsiella pneumoniae and Staphylococcus aureus, respectively, hydroalcohol extract was active against Pseudomonas aeruginosa and Salmonella typhimurium while none of the leaf extracts of the study plant showed inhibition zones against fungal pathogens tested. Conclusion: Multiple solvent extraction approach yielded the phytochemicals which are not yet reported and the antimicrobial activities of phytochemicals acknowledged their medicinal value.
Objective: The present study was aimed to determine the cytotoxicity concentration (CTC50) of different extracts made from the leaf and stem bark of an ethno botanically selected S. pubescens against Human liver carcinoma (Hep G2), Human colon carcinoma (CaCo2) and Human breast cancer (T-47 D) cell lines. Methods: Ethnobotanical survey was done through interviewing traditional medicinal practitioners then a potential herbal plant was selected after a thorough literature survey and its identity was confirmed. The soxhlet extraction method was adopted using five different solvents from leaf and stem bark powders of the study plant and the CTC50 of all the extracts were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay method. Results: Among the different extracts, CTC50 values were significant for stem bark extracts made from methanol (24.51±0.08 µg/ml) against Hep G2, while leaf chloroform extract was promising (57.15±1.75 µg/ml) against CaCo2 and n-hexane extract of leaf exhibited significant value (20.27±1.52 µg/ml) against T-47 D cancer cell lines. Conclusion: The major findings of the present study clearly provides evidence that the leaf and stem bark of S. pubescens possesses the potential anticancer bioactive compound solasodine.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.