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In this study, an environmentally friendly and biodegradable pullulan/graphene bio nanocomposite was prepared and coated on the nanocellulose lm to improve the surface, mechanical, barrier and antibacterial properties. The nanocellulose lms were prepared by using a spray coating of nanocellulose suspension on stainless steel plates. The graphene nanoparticles were prepared by the modi ed Hummers method. The pullulan/graphene bio nanocomposites were prepared by solvent method with the addition of various wt% (0, 0.05, 0.1, 0.2) of graphene with pullulan. The coating was carried out by the roller coating method. Results showed that the increased graphene nanoparticles in pullulan coating increased the opacity, surface hydrophobicity, tensile strength, oxygen transmission rate and watervapour transmission rate of the coated nanocellulose lm. Also, the coated lm showed excellent antibacterial properties against both gram-negative E.coli and gram-positive S.aureus. In this research work, it was concluded that the graphene nanoparticles of 0.2 wt% showed e cient results. The exceptional properties of the pullulan/graphene bio nanocomposite coating on the nanocellulose lm will give a new pathway to high performance food packaging applications.