This study was aimed at assessing the prevalence of bovine viral diarrhea (BVD) in improved dairy cattle of Chitwan, Nawalpur and Rupandehi Districts of Nepal. Altogether 92 blood samples were aseptically collected from jugular vein of cattle and transferred to clot activator tube and transported to National Cattle Research Program, Rampur, Chitwan. This cross sectional study was carried out from Mangsir to Magh of 2075 B.S using purposive sampling Serum separation was done by centrifugation @3500 for 5 minutes. The serum was stored at -20°C till ELISA test was done. Serological analysis was done according to the protocol of ID.vet BVD Ab ELISA kit. Data were analyzed using Ms excel and SPSS version 20.0.The seroprevalence of BVD Virus was found to be 7.76%. Provided that no history of vaccination against BVD in cattle was done in the study area, seropositive was due to natural infection. Geographic location as well as sampling method might have contributed to this result. The result indicated lower prevalence along with no statistical significance to breed and age risk factors undertaken during the research. However significant association was observed between abortion history and seropositivity. Hence, there is a need of further research to identify the disease burden and its impact in the agricultural gross domestic products (AGDP).

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A cross sectional study was conducted to determine the prevalence of dirofilariosis in street dogs of Kathmandu valley and Siddharthanagar, Rupandehi from January 2019 to March 2019. .Blood sample from 155 dogs of Kathmandu Valley and 150 from Siddharthanagar municipality were collected and examined by Wet Smear, Modified Knott Technique and Buffy Coat methods. PCV value and biochemical parameters were analyzed. Data were analyzed to determine the Prevalence of Dirofilaria and correlation of infections with age, sex and biochemical parameters. The prevalence in Kathmandu valley was 0% (0/156) while the prevalence in Siddharthanagar was 19.33%., while the prevalence in direct smear, buffy coat and modified Knott technique were found to be 16%, 16% and 19.33% respectively. The prevalence were significantly (p<0.05) higher in old age and higher in 3-5 years of age i.e. 51.70%..Prevalence was higher in female (21.70%) than male (17.3%).AST and ALT were significantly (p<0.05) increased in infected dogs. Total protein, ALP, BUN, Creatinine level were elevated in the infected dogs.

Background Nematodes of the genus Dirofilaria are widespread vector-borne helminths (VBH) of increasing relevance. Indeed, dirofilariosis is frequently diagnosed in domestic pets, often dogs, associated with a severe clinical condition known as heartworm disease caused by D. immitis . Assessing the distribution pattern of canine dirofilariosis is pivotal to undertake appropriate control measures and define the risk of infection in animals and humans. This study revealed the occurrence of D. immitis in naïve community dogs from Nepal. Methods An epidemiological study was performed in 2019 in Siddharthanagar (Lumbini region, Nepal). A total of 150 blood specimens were examined using direct microscopy, buffy coat centrifugation and modified Knott's method aiming at isolating and identifying Dirofilaria microfilariae. In addition, hematobiochemical parameters, including packed cell volume (PCV), alanine aminotransferase (SGPT), aspartate aminotransferase (SGOT), alkaline phosphate (ALP), creatinine, blood urea nitrogen (BUN), urea and total protein were analyzed, along with a clinical scoring (i.e., body temperature and respiratory rate) of the animals enrolled in this study. Results Out of 150 dogs sampled, 29 (19.3%) had D. immitis microfilariae. The prevalence of the infection varied according to the technique used, ranging from 16.0% to 19.3% based on direct blood smear microscopy and modified Knott method, respectively. The infection rate was significantly ( P <0.05) higher in dogs aged more than 3 years. A positive correlation was observed between SGPT and SGOT and the presence of microfilariae ( P <0.05). Conclusion The current findings revealed the presence of D. immitis in dogs from Nepal, thus providing an explanation for the diagnosis of this VBH is human cases. Further investigations are warranted to accurately define the prevalence of the infection in other pets, instrumentally to reduce the potential burden on the infection in dogs and, accordingly, to control the spread of this parasite to humans.

Backround: Dirofilaria species are important zoonotic filarioid nematodes transmitted by mosquitoes causing a heartworm disease in canines worldwide. Map of the distribution pattern of dirofilariois in Nepalese context is lacking. Methodology: A study was done to access the occurrence of dirofilarioids among stray dogs population in Siddharthanagar, sub-metropolitan city of Lumbini, Nepal. A total of 150 blood specimens were examined using direct microscopy, buffy coat centrifugation and modified Knott's method. Haematobiochemical parameters including packed cell volume (PCV), alanine aminotransferase (SGPT), aspartate aminotransferase (SGOT), alkaline phosphate (ALP), creatinine, blood urea nitrogen (BUN), urea and total protein were evaluated. Furhter, the clinical performance, comprising the body temperature and respiratory rate, were also assessed. Results: The dirofilariod is recognized as Dirofilaria immitis . Overall prevalence of dirofilariosis was reported to be 19.33% (29/150). Meanwhile, microfilariae were noticed to be 16.0, 16.0 and 19.33 % using the direct blood smear microscopy, buffy coat and modified Knott technique, respectively. The infection rate was significantly ( P <0.05) higher in aged dogs with high respiratory rates. The response of sex was non-significant. Among haematobiochemical parameters, only SGPT and SGOT were significantly ( P <0.05) elevated. The body temperature was not altered.Conclusion: Coinciding with the existence of stray dog communities and the recent zoonotic appearance of such filarioids, further investigations including molecular approaches, are urgently needed to accurately differentiate both dirofilarioids and other filarioid nematodes in both pets and humans.

Zoonoses are the common cause of disease occurrence in animals and humans in Bhairahawa. Many zoonotic disease such as Tuberculosis, Rabies, Heartworm, Brucella, Leptospira has been encountered in Veterinary Teaching Hospital, Institute of Agriculture and Animal Science (IAAS), Tribhuvan University from Bhairahawa periphery. We conducted a cross-sectional purposive random survey of total 303 villagers of Siddharthanagar Municipality, Bhairahawa, Rupandehi to study awareness status about zoonosis. Out of total individuals interviewed, 176 (58%) heard about the zoonotic disease and radio/television (37.5%) were the source of information. 66.7% female and 56.9% male knew about zoonosis. 72.9% of youths (30-50 years), 36.6% farmers, 33.33% Dalit and 40.2% Madheshi, 58.6% Hindu, 66.7% Buddhist, 66.7% of Christian knew about zoonosis. 86% of aware people knew about direct transmission rout of zoonotic disease, and 100%, 77.84%, 100%, 100% and 47.72% respondents knew about the contaminated food, milk, meat, bite, and secretion transmission route respectively. Age, education, ethnicity, occupation play highly significant (P<0.05) effect on zoonosis, where religion had significant (P<0.05) effect but sex had no significant relationship. Hence our study suggests that zoonotic disease pose a threat to people but the risk grossly underestimated.

After significant solar eruptions, protons are fired at extremely high speeds, sometimes reaching several thousand kilometer per second (km/s), resulting in solar radiation storms. Solar particle events can be found all around the heliosphere. The cross-correlation analysis along with time series analysis is used to look at how the solar wind and proton flux are related. The parameters used in this work are flux of Solar Energetic Protons (SEP) ranging from >10 MeV to >60 MeV along with speed, density, and pressure of the solar wind parameters. The findings indicate that proton flux (E>10 MeV) shows significant changes just before the storm while flux (E>30 MeV) and (E>60 MeV) doesn’t correlate with solar parameters although during quiet day no significant changes were observed. These finding suggested that SEP can be used as precursor of CME driven storms.

A geomagnetic storm is a major disturbance of Earth’s magnetosphere that occurs when there is a very efficient exchange of energy from the solar wind into the space environment surrounding Earth. During solar and geomagnetic activities, critical frequency of F2 layer (foF2) varies in a great extent. The focus of the current study is on variations in foF2, the critical frequency of the F2 layer, during the intense geomagnetic storm of solar cycle 24 and quiet storm of solar cycle 25. The foF2 data of mid latitude station BC840 (Boulder) in the American region have been used to study geomagnetic and solar wind properties linked with foF2. Time series analysis and Wavelet Coherence analysis have been used to establish the relationship between those parameters. We have found considerable effect of solar and geomagnetic storm disturbances on foF2 during the geomagnetic storm time. The foF2 is enhanced prior to the storm and depletes during recovery phase with no significant changes observed during weak storm. The enhancement or depletion in foF2 may be used as precursor of geomagnetic storms. With long trend analysis and larger database we can make a catalog which can be used to predict the geomagnetic storm and deepen our understanding in impacts of storms on communication signals due to energy injected in ionosphere.