A study was developed to evaluate the influence of triclabendazole (Fasinex) and netobimin (Hapasil) the antigenaemia in sheep naturally infected with Fasciola hepatica during 16 weeks. A sandwich-ELISA (enzyme-linked immunosorbent assay) using a rabbit polyclonal IgG antibody to F. hepatica antigens was employed and the data obtained were compared to those from coprological and indirect-ELISA techniques. Triclabendazole reduced the values of circulating antigens at weeks 2-4 post-treatment and faecal output at weeks 2-8 post-treatment, but antibodies showed positive values until the end of the study. Netobimin did not reduce circulating antigens of the trematode nor egg-excretion; and IgG antibodies did not decrease throughout the study.
The influence of a challenge infection during an acute or chronic primary infection on Fasciola hepatica-coproantigen kinetics was established in rats. Egg-output and IgG humoral responses were also established. Two primary-infected groups were reinfected at different periods, one 4 weeks after primary infection (w.p.i.) (acute phase) and the other 11 w.p.i. (chronic phase). Another group remained without reinfection as a control. Coproantigens were first detected in the group challenged on acute fascioliasis. The highest values of coproantigens were recorded in the group reinfected during acute fascioliasis and the lowest in the group challenged during chronic fascioliasis. The IgG responses to F. hepatica excretory/secretory antigens increased after reinfection. Neither the egg output nor the parasitic burden were significantly different among the three infected groups. Our results indicate that the time of challenge affects the kinetics of coproantigens. Thus, challenge during acute fascioliasis favours the migration of the juvenile flukes from the primary infection to the bile ducts, and is responsible for the earlier presence of coproantigens in these animals. The possible relationship between coproantigen detection and T1 and T2 tegumentary antigens is discussed.
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