Recent studies have suggested that chromosomal aberrations of the MYC gene locus indicate an unfavorable prognosis in diffuse large B-cell lymphoma (DLBCL). However, there have been few reports on MYC translocation in Chinese patients. One hundred and six cases of DLBCLs were analyzed using interphase fluorescent in situ hybridization. Immunophenotyping analysis (CD20, CD3, CD10, Bcl-6, Mum-1) was also performed. MYC translocation was identified in 13 (12.3%) out of 106 cases. All MYC(+) DLBCLs showed a non-germinal center B-cell type. MYC(+) DLBCLs showed significantly poorer overall survival (OS) and progression-free survival, with a median OS and progression-free survival time of 4.7 and 3.2 months, respectively (p < 0.001). Multivariate analysis using a Cox proportional hazard model confirmed that MYC(+) (for OS, Hazards ratio 5.254; 95% CI, 2.354-11.723, p < 0.001) was the strongest independent predictor. DLBCL with MYC translocation is a subgroup of non-germinal center B-cell DLBCL with poor outcome. This may be a clinical characteristic that is specific to Chinese patients. Because only a few patients received rituximab, its usefulness could not be assessed. Future studies with larger numbers of patients are required.

An analytical method was developed for determining free and oligosaccharide-bound sialic acids in bovine colostrum by HPLC. The condition of acid hydrolysis for sample and sialic acids derivatization were investigated. The sialic acids were released from their parent oligosaccharides by acid hydrolysis using 40mL 0.1mol/L sulphuric acid for 100min. They were then derivatized using O-phenylenediamine (OPD) at 70°C for 40 min, the determination of the derivative could be carried out by UV detection. The total content of free and oligosaccharide bound sialic acids in bovine colostrum milk were 941.95μg/mL. The developed method is validated on various dairy products such as bovine milk and milk-based products.

Galactose is found in many oligosaccharides, galactomannans, glycoproteins and glycolipids, which are widely distributed in plants microorganisms and animals. α-Galactosidase (α-Gal) catalyzes the hydrolysis of 1,6-linked α-galactosyl residues and transgalactosylation. α-Gals are classified into four glycoside hydrolases families (GH): 4, 27, 36 and 57. The majority of known α-Gals belongs to GH families 27 and 36.α-Gals are of particular interest in view of their biotechnological applications.

The conditions for purification of soyasaponin by macroporous resin adsorption strategies were analyzed. The results showed that macroporous resin D3520 was a suitable resin for the purification of soyasaponin. Static adsorption assay showed that 20:1 (w/w) D3520/soyasaponin at 40 oC for 2h adsorption were optimal for soyasaponin purification. In the column chromatography, 0.5BV (bed volume)/h flow rate would be suitable to reach higher purity of near 90 % soyasaponin.

Epichlorohydrin crosslinked starch microspheres (ECMs) were synthesized with soluble starch as a raw material and epichlorohydrin as a crosslinker. The characteristics of ECMs were investigated by Scanning Electron Microscopy (SEM) and Fourier Transform InfraRed spectroscopy (FT-IR).The drug loading and in vitro release properties of ECMs were studied using arginine as a model drug. The results indicate that ECMs have a spherical morphology with average diameter about 7μm. The drug loading studies show that after absorption for 1.5 h, the largest amount of drug (drug loading 31mg/g) is loaded when the quantity ratio of ECMs to arginine is 2. In-vitro release studies indicate that the ECMs are effective in controlled releasing arginine over an extended period of about 25 h.