The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (< or = 35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of +/- 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.

No abstract

Diphosphines of the class RPhP(CH2)"PPhR ( = 2-6) have been prepared from the reactions of the corresponding Ph2P(CH2)"PPh2 with alkali metal followed by alkylation. Ultrasound irradiation should be applied in the reductive cleavage stage so as to assure the purity of the final products.Diphosphines of the general type PhRP(CH2)"PRPh are mechanistic study of various transition-metal complexes important chelating reagents for the preparation and

No abstract

No abstract