Through this extensive structure–property study we show that critical micelle concentration correlates with self‐associative hydrogen bond complex formation constant, when combined with outputs from low level, widely accessible, computational models. Herein, we bring together a series of 39 structurally related molecules related by stepwise variation of a hydrogen bond donor–acceptor amphiphilic salt. The self‐associative and corresponding global properties for this family of compounds have been studied in the gas, solid and solution states. Within the solution state, we have shown the type of self‐associated structure present to be solvent dependent. In DMSO, this class of compound show a preference for hydrogen bonded dimer formation, however moving into aqueous solutions the same compounds are found to form larger self‐associated aggregates. This observation has allowed us the unique opportunity to investigate and begin to predict self‐association events at both the molecular and extended aggregate level.

The co-formulation of supramolecular self-associating amphiphiles (SSAs) enhances solution state physicochemical properties and increases efficacy against methicillin-resistant Staphylococcus aureus.