A monoclonal antibody was produced to a Campylobacter-like organism (RMIT 32A) which was isolated from the terminal ileum of a pig with proliferative enteritis. Isotyping of the antibody revealed that it was an IgG2a with kappa light chains. Immunoblots using the antibody against proteinase-K-treated whole cell lysates of RMIT 32A, a selection of Campylobacter species and other enteric bacteria showed that the antibody was specific for RMIT 32A and was directed against the lipopolysaccharide. This antibody can be used for the specific detection of RMIT 32A.
Two growth trials were conducted for 60-days using aquaria tanks (growth trial 1) and 50-days using commercial culture tank (growth trial 2) to assess the potential effect on using phytogenic feed additives (Regavit AquaTM, Ecopharm Hellas, Greece) containing thymol and carvacrol extracted from Oregano plant Origanum Vulgare (OP) by incorporating the OP powder into the diet formulation for trial 1 and top-dressing process of OP liquid for trial 2. The antimicrobial properties of carvacrol and thymol polyphenols were also determined by using minimum inhibitory concentration (MIC) analysis against Vibrio harveyi and Vibrio parahaemolyticus. From the aquaria tank (trial 1), the growth performance, including final average body weight (ABW), percentage weight gain (PWG) and thermal growth coefficient (TGC) of group of shrimp fed with OP were compared to the control treatment. Using top-dressing process (trial 2), the top coating process to the commercial diet enhance the growth performances of shrimp treated with OP compared to the control treatment. From, trial 1, there was clear evidence for elevated hemocyte activity and increased lysozyme in shrimp fed diets supplemented with OP compared to the control treatments. MIC analysis performed in the present study also revealed the ability of OP to inhibit the growth of bacteria at the concentration of 2 × 101 µg mL-1. The results indicate that the inclusion of OP up to 0.6% both in direct inclusion within the diet or using top-dressing process significantly increases the growth and non-specific immune in white shrimps L. vannamei.
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