Background: The cellular response of plants to water-deficits has both economic and evolutionary importance directly affecting plant productivity in agriculture and plant survival in the natural environment. Genes induced by water-deficit stress have been successfully enumerated in plants that are relatively sensitive to cellular dehydration, however we have little knowledge as to the adaptive role of these genes in establishing tolerance to water loss at the cellular level. Our approach to address this problem has been to investigate the genetic responses of plants that are capable of tolerating extremes of dehydration, in particular the desiccation-tolerant bryophyte, Tortula ruralis. To establish a sound basis for characterizing the Tortula genome in regards to desiccation tolerance, we analyzed 10,368 expressed sequence tags (ESTs) from rehydrated rapiddried Tortula gametophytes, a stage previously determined to exhibit the maximum stress induced change in gene expression.

Intra-to multidecadal variation in annual streamflow, precipitation, and temperature over the continental United States are evaluated here through the calculation of Mann-Whitney U statistics over running-time windows of 6-30-yr duration. When this method is demonstrated on time series of nationally averaged annual precipitation and mean temperature during 1896-2001, it reveals that 8 of the 10 wettest years occurred during the last 29 yr of that 106-yr period, and 6 of the 10 warmest years during the last 16. Both of these results indicate highly significant departures from long-term stationarity in U.S. climate at the end of the twentieth century. The effects of increased wetness are primarily evident in the central and eastern United States, while evidence of warmth is found throughout the Rocky Mountain region and in the West. Analysis of annual streamflow records across the United States during 1939-98 shows broadly consistent effects. Initial evidence of the recent wet regime is most apparent in eastern streamflow, which shows a clear pattern of high-ranked mean annual values during the 1970s. Over the midwestern states, a coherent pattern of high-ranked annual flow is found during multidecadal periods beginning during the late 1960s and early 1970s and ending in either 1997 or 1998. During the late 1980s and early 1990s, a significant incidence of low-ranked annual flow conditions throughout the West was roughly coincident with the onset of western warmth during the mid-1980s. Evidence of highly significant transitions to wetter and warmer conditions nationally, and consistent variation in streamflow analyses, suggests that increased hydrological surplus in the central and eastern United States and increased hydrological deficit in the West may be representative of the initial stages of climate change over the continental United States.

Regional changes in California surface temperatures over the last 80 years are analyzed using station data from the US Historical Climate Network and the National Weather Service Cooperative Network. Statistical analyses using annual and seasonal temperature data over the last 80 years show distinctly different spatial and temporal patterns in trends of maximum temperature (Tmax) compared to trends of minimum temperature (Tmin). For trends computed between 1918 and 2006, the rate of warming in Tmin is greater than that of Tmax. Trends computed since 1970 show an amplified warming rate compared to trends computed from 1918, and the rate of warming is comparable between Tmin and Tmax. This is especially true in the southern deserts, where warming trends during spring (March-May) are exceptionally large. While observations show coherent statewide positive trends in Tmin, trends in Tmax vary on finer spatial and temporal scales. Accompanying the observed statewide warming from 1970 to 2006, regional cooling trends in Tmax are observed during winter and summer. These signatures of regional temperature change suggest that a collection of different forcing mechanisms or feedback processes must be present to produce these responses.

In a recent study the authors developed the first regionally averaged, transnational records of snowpack and streamflow for the Andes between 30° and 37°S using Chilean and Argentinean data. That study was mainly intended to evaluate the relationships between the interannual variations in the regional snowpack record and large-scale atmospheric variables and indices. Here the focus is on the main intra- to multidecadal variations in updated records of winter snowpack and mean annual river flows. River discharges show similar temporal variations on both sides of the Andes with extreme dry conditions concentrated between the mid-1940s and 1976/77 and extreme wet conditions peaking between the late 1970s and the 1980s. A regional streamflow composite (1906–2007) has a nonsignificant negative trend but significant regime shifts in 1945, when mean levels dropped 31%, and in 1977 when they increased 28%. These events coincide almost exactly with well-known shifts in the Pacific decadal oscillation (PDO). The analyses are preliminary but suggest a PDO influence on the low-frequency modes of hydroclimatic variability in the study area. Analyses of the magnitude of 5–20-yr moving windows in the regional streamflow composite indicate that the most significant concentration of high (low) discharges occurred between 1977 and 1987 (1954 and 1971). Snowpack series show a more heterogeneous pattern of variations on a local basis but when aggregated into a regional series (1951–2008) they share remarkable similarities with river flows. However, the snowpack composite has a stronger year-to-year variability, a slight positive trend, and no significant regime shifts.