Similar to Neisseria gonorrhoeae, tetracycline-resistant isolates of N. meningitidis, Kingella denitrificans, and Eikenella corrodens contained 25.2-megadalton plasmids carrying the TetM determinant. In contrast, tetracycline-resistant N. subflava biovar perflava-N. sicca and N. mucosa isolates carried the TetM determinant in the chromosome.

Background: The majority of research to date on the links between wellbeing and green spaces comes from cross-sectional studies. Purpose: Shmapped is an app which allows for the collection of wellbeing and location data live in the field and acts as a novel dual data collection tool and wellbeing intervention which prompts users to notice the good things about their surroundings. Methods: We describe the process of developing Shmapped from storyboarding, budgeting and timescales, selecting a developer, drawing up data protection plans, collaborating with developers and end-user testers to ultimately publishing Shmapped. Results and Conclusions: The development process and end-user testing resulted in a highly functional app. Limitations and future uses of such novel dual data collection and intervention apps are discussed and recommendations made for prospective developers and researchers.

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As the number of feral cats continues to increase, land managers, public health officials, and private citizens are voicing concerns about how to address the nuisance and public health impacts, as well as animal welfare concerns, that feral cats create. Trap-neuter-release programs aimed at reducing feral cat populations without euthanasia are gaining popularity in the United States. But do they work? Authors Mark Hostetler, Samantha M. Wisely, Steve Johnson, Elizabeth F. Pienaar, and Martin Main discuss the pros and cons of trap-neuter-release programs in this 8-page fact sheet published by the UF/IFAS Department of Wildlife Ecology and Conservation. https://edis.ifas.ufl.edu/uw468

Standard and experimental tests were used by a reference diagnostic laboratory to determine the identity of 182 "suspected" Neisseria gonorrhoeae isolates submitted by state health departments because of inconclusive laboratory results. More than 97% of these cultures were subsequently identified by a rapid microcarbohydrate test in conjunction with confirmatory immunological procedures. The experimental rapid slide agglutination test using rough-lipopolysaccharide antibody, the Phadebact co-agglutination test, and fluorescent antibody test identified 49.3 to 94.1% of these cultures. Because of frequent problems with carbohydrate utilization, Neisseria meningitidis and Branhamella catarrhalis were the two microorganisms most often confused with N. gonorrhoeae by submitting laboratories.