Investigation of sequence variation in common inbred mouse strains has revealed a segmented pattern in which regions of high and low variant density are intermixed. Furthermore, it has been suggested that allelic strain distribution patterns also occur in well defined blocks and consequently could be used to map quantitative trait loci (QTL) in comparisons between inbred strains. We report a detailed analysis of polymorphism distribution in multiple inbred mouse strains over a 4.8-megabase region containing a QTL influencing anxiety. Our analysis indicates that it is only partly true that the genomes of inbred strains exist as a patchwork of segments of sequence identity and difference. We show that the definition of haplotype blocks is not robust and that methods for QTL mapping may fail if they assume a simple block-like structure.

No abstract

Aims and MethodTo examine the quality of information concerning depression available on the internet, identify factors associated with ‘good’ quality, and develop a simple-to-use instrument for assessing websites on depression. The Depression-Website Content Checklist was developed and compared with a previously validated, yet too complex, scale. Websites were assessed using both instruments.ResultsGood quality information is more likely to be found on websites provided by governmental, professional and charitable organisations. The differences we observed in the median scores for these websites using the Depression-Website Content Checklist are significant at the 0.05 level (Ctot; Mann–Whitney, U=24.00; P=0.013). The Depression-Website Content Checklist is a valid and reliable user-friendly tool.Clinical ImplicationsPatients can be directed towards better quality information by diverting them to public and non-sponsored websites. Clinicians can use the Depression-Website Content Checklist to determine website quality.

Summary There has been considerable interest in how doctors break bad news, with calls from within the profession and from patients for doctors to improve their communication skills. In order to aid clinical training and assessment of the skills used in breaking bad news there is a need for a reliable, practical and valid, structured rating schedule. Such a rating schedule was compiled from agreed criteria in the literature. Video-taped recordings of simulated consultations breaking bad news were independently assessed by three raters using the schedule and compared to three experts who gave global ratings. The primary outcome measures were internal consistency of the schedule and level of agreement between raters. The internal consistency was high with a Cronbach's alpha of 0.93. Agreement between raters using the schedule was moderate to good. The majority of the variation in scores was due to the differences in skills demonstrated in the interviews. The agreement between raters not using the schedule was poor. The BAS provides a simple to use, reliable, and consistent rating schedule for assessing skills used in breaking bad news. It could be a valuable aid to teaching this difficult task.