The causes of toxemia of pregnancy are not fully understood. This project examines rat kidneys to determine if a mouse anti‐renin antibody will cross‐react rat renin‐producing cells in the rat. This will allow further study female reproductive tissues in the rat to examine for renin production. Renin is known as hormone produced by the juxtaglomerular cells of the afferent arteriole of the kidney in response low blood pressure. Also, renin is an immediate downstream target of Class I Hox gene regulation, important in embryonic development. In mice, renin is expressed in embryonic renal, adrenal and gonadal arteries, the adrenal glands, and tissue associated with embryonic pronephric and mesonephric kidneys. Using a renin/GFP reporter in transgenic mice, renin is expressed in placental giant trophoblast cells and uterine/ovarian artery cells of the tunica adventitia. In RCS rats, renin antibody stain that used anti‐rat renin and peroxidase labeled placental giant trophoblast cells and cells of the tunica adventitia of the uterine/ovarian artery. This project examines rat kidney tissue labeled by indirect immuno‐fluorescence with anti‐mouse renin antibody and fluorescent conjugate. Cells in the juxtaglomerular region of the kidney were renin positive. This demonstrates that anti‐mouse renin antibody can dependably label regions in the rat kidney that are known for renin production. This will allow for further studies to determine renin location in rat female reproductive tissues. Dr. Craig Jones of Roswell Park Cancer Institute generously donated mouse renin antibody and lab space for stain procedures. D'Youville College Faculty Council and Department of Math & Natural Sciences supported work.
Renin is an important kidney vasculature‐produced hormone that is released with a drop in the vascular flow rate through the glomerulus. It activates angiotensinogen, which is altered to angiotensin I, and altered again in lung tissue to angiotensin II, causing a release of aldosterone from the adrenal cortex and an increase in blood pressure, thereby increasing the vascular flow rate through the glomerulus. Renin is also a hox gene product in the mouse, in the abdominal region, found in fetal paired vessels of the abdominal aorta, supplying adrenal glands, kidneys, and gonads. After birth renin is still present in mouse ovarian/uterine artery, but not in the testicular artery. It is also present in giant trophoblast cells of the mouse placenta. Our lab has shown that renin is produced in rat female reproductive tissues, in the ovarian/uterine arteries and giant trophoblast cells, using anti‐mouse renin antibodies. This study examines the sites of renin immunohistochemical staining in rat female reproductive tissues during each stage of the estrus cycle. Anti‐mouse renin antibody generously donated by Dr. Kenneth Gross of RPCI. Research supported by DYC faculty research committee funding.
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