Background: Fibromyalgia engulfs patients in a downward, reinforcing cycle of unrestorative sleep, chronic pain, fatigue, inactivity, and depression. In this study we tested whether a mostly raw vegetarian diet would significantly improve fibromyalgia symptoms.

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Changes in chemical composition, water content, respiration, and ability to survive rapid dehydration in dry air were studied in nematodes, Aphelenchus avenae, recovering from anhydrobiosis, in water. Water content rises rapidly, from about 0.02 mg H,O/mg dry weight to 2.0 mglmg after five minutes and 2.9 mglmg after four hours. Glycogen increases from 5 pglmg to about 25 pglmg after one hour. Glycerol and trehalose fall from 45 pglmg and 125 pglmg respectively to undetectable levels and 70 pglmg after four hours. Oxygen uptake rises from about 25 nllmglmin after ten minutes to 450 nllmgl min after 1.5 hours and then falls to about 150 nllmg min after four hours. Lipid content declines from 124 pglmg to 51 pglmg after 24 hours. Correlations were observed between survival in dry air and glycerol, trehalose, and water contents.

The mean lethal temperature of Melampus bidentatus Say ranged from −5 °C in July to −13 °C in December. The percentage of tissue water frozen measured at the mean lethal temperatures was 3% for snails collected in the summer and 74.9% for snails collected in the winter. The supercooling point of the whole snails ranged from −9 °C in September to −6.4 °C in February. The presence of an ice nucleator is indicated by an increase in the supercooling point of the haemolymph from −11.5 °C in the fall to −7.5 °C in the winter. Water content in the snails varied from 2.6 mg water/mg dry weight to 3.6 mg water/mg dry weight; however, there was no correlation between low water contents and an increase in freezing tolerance. Whole animal glycerol contents ranged from 1.14 to 2.96 μmol/g fresh weight and proline contents ranged from 1.20 to 2.19 μmol/g fresh weight. Sorbitol and trehalose were not detected.