Sequence analysis of the vaccinia virus strain Western Reserve genome revealed the presence of an open reading frame (ORF), SalLAR, which has the potential to encode a transmembrane glycoprotein with homology to C-type animal lectins (

During the preparation of the manuscript, an error occurred in the wording of the final sentence of the abstract. The correct sentence appears below.To our knowledge, these results demonstrate a previously unrecognized function for GATA4 as a regulator of cardiac angiogenesis through a nonhypoxic, load, and/or disease-responsive mechanism.The JCI regrets the error.

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GATA6 defines endoderm fate by controlling chromatin accessibility during differentiation of human-induced pluripotent stem cells Graphical abstract Highlights d Newly accessible chromatin in definitive endoderm is enriched for GATA motifs d Definitive endoderm patterning is significantly perturbed in GATA6 À/À cells d GATA6 interacts with multiple chromatin remodeling complexes d GATA6-dependent patterning commonly precedes the onset of gene expression

HNF-4α is a transcription factor of the nuclear hormone receptor family that is expressed in the hepatic diverticulum at the onset of liver development. Mouse embryos lacking HNF-4α fail to complete gastrulation due to dysfunction of the visceral endoderm. This early embryonic lethality has so far prevented any analyses of the contribution of HNF-4α toward liver development and hepatocyte differentiation. However, we have shown that complementation ofHNF-4 α−/−embryos with a tetraploid embryo-derived wild-type visceral endoderm rescues this early developmental arrest and allowsHNF-4 α−/−embryos to proceed normally through midgestation stages of development. Examination of these rescued embryos revealed that HNF-4α was dispensable for specification and early development of the liver. However,HNF-4α−/− fetal livers failed to express a large array of genes whose expression in differentiated hepatocytes is essential for a functional hepatic parenchyma, including genes encoding several apolipoproteins, metabolic proteins, and serum factors. In addition, we have demonstrated that HNF-4α is essential for expression of the transcription factors HNF-1α and PXR within the fetal liver. We therefore conclude that HNF-4α is both essential for hepatocyte differentiation during mammalian liver development and also crucial for metabolic regulation and liver function.