Influenza virus induces apoptosis in infected cells to promote viral replication by manipulating the host cell death signaling pathway. Although some Bcl-2 family proteins play a role in the replication of influenza A virus (IAV), the role of cell death pathways in the viral replication cycle is unclear. We investigated whether deficiency of the proapoptotic Bcl-2 family protein, Bik, plays a role in IAV replication. IAV replication was attenuated in mouse airway epithelial cells (MAECs) from bik 2/2 compared with bik Clinical RelevanceThis study identifies Bik as a novel host cell protein that plays an important role in influenza A virus replication. Because Bik promotes influenza A infection through caspase 3 activation and proper cytoplasmic export of viral RNPs, inhibiting Bik expression or caspase 3 activation may be a novel therapeutic approach to reducing influenza A infection.Influenza A virus (IAV) infection is associated with 36,000 deaths and 226,000 hospitalizations annually, with losses in the tens of billions of dollars to the economy of the United States (1). Influenza infections pose serious challenges because of the lack of effective therapeutic interventions and the rapid evolution of viral genomes toward resistance. Therefore, understanding the molecular mechanism by which IAV replicates will help identify targets for effective antiviral drugs that are less susceptible to resistance.Influenza virus induces apoptosis in infected epithelial, lymphocyte, and phagocytic cells (2) and mainly damages epithelial cells of the human respiratory tract (3). Although apoptosis is required for viral replication (3), how the cell death pathways interplay with viral replication is
This report reviews the current state-of-the-art applied approaches on automated tools, services and workflows for extracting information from images of natural history specimens and their labels. We consider the potential for repurposing existing tools, including workflow management systems; and areas where more development is required. This paper was written as part of the SYNTHESYS+ project for software development teams and informatics teams working on new software-based approaches to improve mass digitisation of natural history specimens.
This report investigates the current state of physical (mechanical) robotics, automated warehousing approaches and assistive technologies in relation to the storage, handling and processing (particularly digitisation) of natural history collections. Robotics can sound futuristic, however we provide case studies that show many and growing examples of physical automation in the natural history and cultural heritage sectors, including barcodes and conveyor belts for digitisation; robots that handle multiple vials for molecular and genetic work; robots for use in in display or exhibition contexts; and automated warehousing of library collections. We provide a non-exhaustive example of an end to end workflow of storage, retrieval and processing and discuss aspects of the tools and challenges relevant to these stages. The Distributed System of Scientific Collections (DiSSCo), a new Research Infrastructure for natural science collections, should build on this, leading a future programme of pilots that develop understanding of independent stages, and can be connected to make progress towards end-to-end solutions. Robots, or automated systems, excel at repetitive tasks, and are developing rapidly to be able to handle more complex object types, at lower cost. High volume, high variety of objects, and considerations such as fragility are not unique to the natural history sector - they apply for example to major retail operations - however natural history collections do offer some of the more extreme examples of these challenges, and in particular are not replaceable. Increased consistency of storage units is likely to be a critical factor in enabling automated handling in future, as well as looking at automation possibilities when new collections storage spaces are developed and built. Engagement with industry and subject matter experts has been patchy and again we recommend that DiSSCo help to ensure a joined up engagement with the right incentives in place, and with clear communication of requirements and challenges for shared R&D. When examining return on investment for particular automation, collections-holding institutions need to consider not only time and cost of automation compared to human labour, but wider factors including: health and safety such as physical environment and repetitve strain injury; security; quality and consistency of outputs; degree of criticality in response times (e.g. if digitising on demand); effective use of spaces; and freeing up staff to conduct other tasks. Purely software-based automation is outside the scope of this report, but is also in increasing use and has enormous potential, for example to transform the extraction of label and specimen data at scale from images. The challenges of managing and digitising collections at scale under DiSSCo are likely to require a combination of hardware and software automation approaches.
There has been little work to compare and understand the operating costs of digitisation using a standardised approach. This paper discusses a first attempt at gathering digitisation cost information from multiple institutions and analysing the data. This paper has been written: for other digitisation managers who want to breakdown and compare project costs; as a potential baseline for future digitisation projects; as a starting point for prioritising research and development to reduce digitisation costs.
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