It has been shown that bacteria in a postantibiotic (PA) phase exposed to subinhibitory concentrations (sub-MICs) of antibiotics show a long delay before regrowth. This effect has been named the PA sub-MIC effect (PA SME). In the present study, we have used a new method to demonstrate this phenomenon. A computerized incubator for bacteria, Bioscreen C (Lab Systems, Helsinki, Finland), which incubates the bacteria, measures growth continuously by vertical photometry, processes the data, and provides a printout of the results was used. With this method, one may easily test several antibiotics against different bacteria for PA effects (PAEs), PA SMEs, and SMEs. In this study, the effects of benzylpenicillin against 1-hemolytic streptococci and pneumococci were examined. The bacteria were exposed to 2, 10, or 5Ox MIC for 2 h, washed and diluted, incubated in the Bioscreen C incubator, and then exposed to 0.1 to 0.9x MIC. The regrowth was monitored for 20 h. The PAE was calculated as the difference in the time required for the exposed and unexposed bacteria to grow to a defined point (A50) on the absorbance curve. A50 was defined as 50% of the maximum absorbance for the control cultures. The PA SMEs were calculated as the difference in the time required for the reexposed cultures and the unexposed controls to reach Aso. The PAEs ranged between 0.6 and 3.2 h and varied little with the concentration used for the induction of the PAEs. At 0.2x MIC, the PA SMEs were 2 to 3 h longer than the PAEs. Higher sub-MICs increased this delay before regrowth. Most cultures exposed to sub-MICs alone were only slightly affected compared with the controls.Earlier studies and clinical experience have shown that in the treatment of streptococcal and pneumococcal infections, intermittent doses of penicillin are successful even if concentrations in serum and tissues fall below the MIC for the bacteria for long intervals (11, 24). These results may partly be explained by the so-called postantibiotic (PA) effect (PAE), i.e., the suppression of bacterial growth that persists after limited exposure to an antibiotic (3). When intermittent dosing is applied in clinical practice, however, there is a gradual decrease in the antibiotic concentration in which suprainhibitory concentrations will often be followed by a period of subinhibitory concentrations (sub-MICs). The effects of these sub-MIC levels on bacteria may be an additional explanation for the success of intermittent dosage schedules. We have shown earlier that in certain antibioticbacterium combinations, when a PAE is found, there is a long delay before regrowth when the bacteria are reexposed to sub-MICs during the PA phase (PA sub-MIC effect [PA SME]). The PA SMEs have generally been found to be more pronounced than the direct effect of sub-MICs on bacteria not previously exposed to antibiotics (13)(14)(15). In these experiments, we used experimental and control cultures in broth from which serial subcultures were transferred onto solid media, and the numbers of CFUs were counted. Ho...

The pharmacodynamic effects of benzylpenicillin against Streptococcus pyogenes were studied in a new in vitro kinetic model in which bacterial outflow was prevented by a filter membrane. Following the administration of an initial dose of antibiotic, decreasing concentrations were produced by dilution of the medium. A magnetic stirrer was placed above the filter to avoid blockage of the membrane and to ensure homogeneous mixing of the culture. Repeated samplings were easily provided through a silicon diaphragm. Streptococci were exposed to a single dose corresponding to 1.5, 10, 100, or 500 x the MIC of benzylpenicillin and also to an initial concentration of 10 x the MIC of benzylpenicillin, followed by exposure to a repeated dose after 8 h yielding 10 or 1.5 x the MIC. Experiments were also performed with 10 x the MIC of benzylpenicillin with a half-life of 3 h or an initial half-life of 1.1 h that was altered to 3 h at the time point at which the antibiotic concentrations and MIC intersected. Bacterial killing and regrowth were followed by determining viable counts. The post-MIC effect (PME) was defined as the difference in time for the numbers of CFU in the culture vessel to increase 1 log10 CFU/ml, calculated from the numbers obtained at the time when the antibiotic concentration had declined to the MIC, and the corresponding time for a control culture, grown in a glass tube without antibiotic, to increase 1 log10 CFU/ml. To determine how much of the PME was attributable to subinhibitory concentrations, penicillinase was added to a part of the culture drawn from the flask at the time when the antibiotic concentration had fallen to the MIC. The longest PME was found in the experiments in which the half-life was extended from 1.1 to 3 h at the MIC. This illustrated that sub-MICs are sufficient to prevent regrowth. However, when the half-life was 3 h during the whole experiment, the PME was shorter, indicating that when concentrations decline slowly penicillin-binding proteins will already be present in amounts sufficient for regrowth at the time when the MIC is reached. The PME may prove to be a more reliable factor than the in vitro postantibiotic effect or postantibiotic sub-MIC effect for the design of optimal dosing schedules, since the PME, like the in vivo postantibiotic effect, includes the effects of subinhibitory concentrations and therefore better reflects the clinical situation with fluctuating antibiotic concentrations.

SUMMARYThe redispersal factor for bacteria-carrying particles from a contaminated floor was determined after mopping, blowing and walking activity. Walking gave the highest redispersal factor, 3-5 x 103 mr1, which was three times higher than for blowing and 17 times higher than for mopping. The mean die-away rate for the bacteria-carrying particles used was 1.9/h without ventilation and 14.3/h with ventilation. It was calculated that in the operating rooms less than 15 % of the bacteria found in the air were redispersed floor bacteria.

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