Cervical cancer is the fourth leading cause of cancer mortality in women worldwide.Beetroot (Beta vulgaris L.) has bioactive compounds that can inhibit the progression of different types of cancer. To analyze the antiproliferative effects of beet leaf and root extracts, we performed MTT, clonogenic survival, cell cycle analysis, Annexin/PI labeling, and western blotting. Here, we report that 10 and 100 μg/ml of root and leaf extracts decreased cell viability and potentiated rapamycin and cisplatin effects while decreased the number of large colonies, especially at 10 μg/ml (293.6 of control vs. 200.0 of leaf extract, p = .0059; 138.6 of root extract, p = .0002). After 48 hr, 100 μg/ml of both extracts led to increased sub-G1 and G0/G1 populations. In accordance, 100 μg/ml of root extract induced early apoptosis (mean = 0.64 control vs. 1.56 root; p = .048) and decreased cell size (p < .0001). Both extracts decreased phosphorylation and expression of mechanistic Target of Rapamycin (mTOR) signaling, especially by inhibiting ribosomal protein S6 (S6) phosphorylation, increasing cleaved poly(ADP-ribose) polysomerase 1 (PARP1) and Bcl-2-like protein 11 (BIM), and decreasing cyclin D1 expression, which regulates cell cycle progression. Here, we demonstrate that beetroot and leaf extracts could be an efficient strategy against cervical cancer.
Cervical cancer is the third cause of cancer between women in 2018. Beetroot has bioactive compounds that have the ability to inhibit the progression of different types of cancer cells. The present study analyzed the possible functional effects of leaf and root extracts of beet as anti-tumor agents in cultures of HeLa cervical cancer cells, testing viability (MTT) and colony formation. The results showed a reduction in viability at concentrations of 10 and 100 ppm and in the diameter of the colonies at the concentration of 10 ppm. This work may contribute for new therapeutic and adjuvant approaches for cervical cancer treatment using beet derived compounds and extracts.
Construction civil is one of the sectors that generate more solid waste, as well as there are several studies looking for solutions to best manage and reuse such material. The waste foundry sand (WFS) is a waste generated in large quantity by foundry industries. The application of this waste in construction is a way to reduce the environmental impact and natural resources exploration. On this wise, this research on such topics is to propose the WFS application in landfill and in pavements.
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