Ambient Assisted Living (AAL) is an emerging multi-disciplinary field aiming at exploiting information and communication technologies in personal healthcare and telehealth systems for countering the effects of growing elderly population. AAL systems are developed for personalized, adaptive, and anticipatory requirements, necessitating high quality-of-service to achieve interoperability, usability, security, and accuracy. The aim of this paper is to provide a comprehensive review of the AAL field with a focus on healthcare frameworks, platforms, standards, and quality attributes. To achieve this, we conducted a literature survey of state-of-the-art AAL frameworks, systems and platforms to identify the essential aspects of AAL systems and investigate the critical issues from the design, technology, quality-of-service, and user experience perspectives. In addition, we conducted an email-based survey for collecting usage data and current status of contemporary AAL systems. We found that most AAL systems are confined to a limited set of features ignoring many of the essential AAL system aspects. Standards and technologies are used in a limited and isolated manner, while quality attributes are often addressed insufficiently. In conclusion, we found that more inter-organizational collaboration, user-centered studies, increased standardization efforts, and a focus on open systems is needed to achieve more interoperable and synergetic AAL solutions.

Single-molecule spintronics investigates electron transport through magnetic molecules that have an internal spin degree of freedom. To understand and control these individual molecules it is important to read their spin state. For unpaired spins, the Kondo effect has been observed as a low-temperature anomaly at small voltages. Here, we show that a coupled spin pair in a single magnetic molecule can be detected and that a bias voltage can be used to switch between two states of the molecule. In particular, we use the mechanically controlled break-junction technique to measure electronic transport through a single-molecule junction containing two coupled spin centres that are confined on two Co(2+) ions. Spin-orbit configuration interaction methods are used to calculate the combined spin system, where the ground state is found to be a pseudo-singlet and the first excitations behave as a pseudo-triplet. Experimentally, these states can be assigned to the absence and occurrence of a Kondo-like zero-bias anomaly in the low-temperature conductance data, respectively. By applying finite bias, we can repeatedly switch between the pseudo-singlet state and the pseudo-triplet state.

Despite much evidence for phosphatidylinositol phosphate (PIP)-triggered signaling pathways in the nucleus, there is little understanding of how the levels and activities of these proteins are regulated. As a first step to elucidating this problem, we determined whether phosphatase and tensin homolog deleted on chromosome 10 (PTEN) enters the nucleus by passive diffusion or active transport. We expressed various PTEN fusion proteins in tsBN2, HeLa, LNCaP, and U87MG cells and determined that the largest PTEN fusion proteins showed little or no nuclear localization. Because diffusion through nuclear pores is limited to proteins of 60,000 Da or less, this suggests that nuclear translocation of PTEN occurs via diffusion. We examined PTEN mutants, seeking to identify a nuclear localization signal (NLS) for PTEN. Mutation of K13 and R14 decreased nuclear localization, but these amino acids do not appear to be part of an NLS. We used fluorescence recovery after photobleaching (FRAP) to demonstrate that GFP-PTEN can passively pass through nuclear pores. Diffusion in the cytoplasm is retarded for the PTEN mutants that show reduced nuclear localization. We conclude that PTEN enters the nucleus by diffusion. In addition, sequestration of PTEN in the cytoplasm likely limits PTEN nuclear translocation.

Introduction Preoperative planning is an essential part of total hip arthroplasty (THA). It facilitates the surgical procedure, helps to provide the correct implant size and aims at restoring biomechanical conditions. In recent times, surgeons rely more and more on digital templating techniques. Although the conversion to picture archiving and communication system had many positive effects, there are still problems that have to be taken into consideration. Objectives The core objective was to evaluate the impact of the planners’ experience on the accuracy of predicting component size in digital preoperative templating of THA. In addition, the influence of overweight and obesity (according to WHO-criteria), patient’s sex and component design on the accuracy of preoperative planning have been analysed. Materials and methods The retrospective study included 632 consecutive patients who had primary uncemented THA. Digital templating was done using “syngo—EndoMap” software by Siemens Medical Solutions AG. Mann–Whitney U test and Kruskal–Wallis test have been used for statistical analysis. The accuracy of predicting component size has been evaluated by comparing preoperative planned sizes with implanted sizes as documented by the surgeons. The planner’s experience was tested by comparing the reliability of preoperative planning done by senior surgeons or residents. The influence of BMI on predicting component size has been tested by comparing the accuracy of digital templating between different groups of BMI according to WHO-criteria. The same procedure has been done for evaluating the impact of patient´s sex and component design. Results The implant size was predicted exactly in 42% for the femoral and in 37% for the acetabular component. 87% of the femoral components and 78% of the acetabular cups were accurate within one size. Digital templating of femoral implant size was significantly more reliable when done by a senior surgeon. No difference was found for the acetabular component sizes. The BMI also had an impact on estimating the correct femoral implant size. In overweight patients, planning was significantly more inaccurate than normal weight people. Differences were seen in obese patients. However, these were not significant. Accuracy of acetabular components was not affected. The design of the prostheses and the patient’s sex had no influence on predicting component size. Conclusions Inexperience and overweight are factors that correlate with inaccuracy of preoperative digital templating in femoral components, whereas acetabular components seem to be independent of these factors.

We carry out experiments on single-molecule junctions at low temperatures, using the mechanically controlled break junction technique. Analyzing the results received with more than ten different molecules the nature of the first peak in the differential conductance spectra is elucidated. We observe an electronic transition with a vibronic fine structure, which is most frequently smeared out and forms a broad peak. In the usual parameter range we find strong indications that additionally fluctuations become active even at low temperatures. We conclude that the electrical field feeds instabilities, which are triggered by the onset of current. This is underscored by noise measurements that show strong anomalies at the onset of charge transport.

NLP algorithms are increasingly used in computational social science to take linguistic observations and predict outcomes like human preferences or actions. Making these social models transparent and interpretable often requires identifying features in the input that predict outcomes while also controlling for potential confounds. We formalize this need as a new task: inducing a lexicon that is predictive of a set of target variables yet uncorrelated to a set of confounding variables. We introduce two deep learning algorithms for the task. The first uses a bifurcated architecture to separate the explanatory power of the text and confounds. The second uses an adversarial discriminator to force confound-invariant text encodings. Both elicit lexicons from learned weights and attentional scores. We use them to induce lexicons that are predictive of timely responses to consumer complaints (controlling for product), enrollment from course descriptions (controlling for subject), and sales from product descriptions (controlling for seller). In each domain our algorithms pick words that are associated with narrative persuasion; more predictive and less confound-related than those of standard feature weighting and lexicon induction techniques like regression and log odds.

The major-histocompatibility-complex protein UAP56 (BAT1) is a DEAD-box helicase that is deposited on mRNA during splicing. UAP56 is retained on spliced mRNA in an exon junction complex (EJC) or, alternatively, with the TREX complex at the 5Ј end, where it might facilitate the export of the spliced mRNA to the cytoplasm. Using confocal microscopy, UAP56 was found to be concentrated in RNA-splicing speckled domains of nuclei but was also enriched in adjacent nuclear regions, sites at which most mRNA transcription and splicing occur. At speckled domains, UAP56 was in complexes with the RNA-splicing and -export protein SRm160, and, as measured by FRAP, was in a dynamic binding equilibrium. The application of an in vitro FRAP assay, in which fluorescent nuclear proteins are photobleached in digitonin-extracted cells, revealed that the equilibrium binding of UAP56 in complexes at speckled domains was directly regulated by ATP binding. This was confirmed using a point mutant of UAP56 that did not bind ATP. Point mutation of UAP56 to eliminate ATP binding did not affect RNA splicing, but strongly inhibited the export of mRNA to the cytoplasm. Supplementary material available online at

The Ser-Arg (SR)-related protein SRm160 is a coactivator of premRNA splicing. It bridges splicing factors located at the 5 splice site, branch site, and 3 splice site. Recently, SRm160 has also been shown to be involved in mRNA export as part of an exon-junction complex. SRm160 is highly concentrated in splicing speckles but is also present in long branched intranuclear tracks connecting splicing speckles with sites at the nuclear lamina. In this study we identified domains of SRm160 important for spatial targeting within the nucleus and for binding to the nuclear matrix. Using a series of FLAG-and enhanced GFP-conjugated deletion mutants we found two contiguous sequences that independently target SRm160 to nuclear matrix sites at splicing speckled domains: amino acids 300 -350 and 351-688. Constructs containing amino acids 300 -350 were also targeted to sites peripheral to speckled domains where most mRNA originate subsequent to splicing. Sequences from the N-terminal domain localized proteins to the nuclear lamina near sites where mRNA leaves the nucleus.RNA splicing ͉ RNA export ͉ speckled domains ͉ SRm300 S ince the discovery of RNA splicing (1), its mechanism has been elucidated by the clever use of in vitro assays (2). Simple precursor RNAs, usually with one small intron, are added to a nuclear extract. After the addition of ATP, spliceosomal complexes form, and introns are removed slowly. In marked contrast, native RNA splicing in cells is far more rapid and efficient, capable of processing more complicated substrates. Precursor RNAs as large as 80,780 bases with as many as 175 introns (3) are rapidly spliced, often in complicated but precise alternative patterns. The very rapid splicing seen in vivo likely reflects, in part, the accurate positioning of splicing substrates and factors by the highly ordered architecture of the nucleus. Most RNA splicing factors are concentrated in subnuclear structures that appear as speckled domains when visualized by immunofluorescence microscopy (4). When seen by electron microscopy, these correspond to interchromatin granule clusters (5) that are surrounded by regions rich in the perichromatin fibrils that contain many new transcripts (5, 6). A majority of these transcripts are spliced at or near speckled domains (7), and mechanisms have been described for recruiting splicing factors from these domains to newly activated genes (8,9).Evidence that the nuclear matrix has a critical role in RNA splicing has emerged from studies examining cells expressing a ␤-globin pre-mRNA splicing construct (10,11). This precursor remains associated with the nuclear matrix after its isolation and is spliced rapidly after addition of the ATP (11). In contrast to conventional in vitro splicing reactions, splicing in situ on nuclear matrix preparations occurs without a lag period, indicating that spliceosomal commitment complexes are preassembled and fully functional.Two strong candidates for factors that might couple splicing components are Ser-Arg (SR)-related matrix protein of 160 kDa (...