Enzyme recycling is essential for the development of large-scale
enzyme-catalyzed biotransformations. Recycling is most convenient using enzymes immobilized on solid supports. Although
immobilization on solid supports has been pursued since the
1950s, there are no general rules for selecting the best support
for a given application. The commercial products EUPERGIT
C and EUPERGIT C 250 L have been used for a wide variety
of different enzymes and reactions. The present review draws
up a comprehensive application profile of both EUPERGIT
carriers. The reader gets (a) examples of biotransformations
using oxidoreductases, transferases, hydrolases and lyases
immobilized on EUPERGIT; (b) key data of the biotransformations, i.e., scale, yield, purity, and enantiomeric excess; (c)
efficiency of the immobilization (% immobilized activity); (d)
where appropriate, operational stability of the immobilized
enzyme preparations, that is, number of cycles, residual activity;
(e) specific advantages of the immobilized enzyme over the free
enzyme apart from enzyme recycling, for example, improved
stability and selectivity. Thus, the present review can serve as
a guideline when selecting a resin for enzyme immobilization.
Literature published between 1985 and 2000 is covered.
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