Hypertension is a common disorder of multifactorial origin that constitutes a major risk factor for cardiovascular events such as stroke and myocardial infarction. Previous studies demonstrated an enhanced signal transduction via pertussis toxin-sensitive G proteins in lymphoblasts and fibroblasts from selected patients with essential hypertension. We have detected a novel polymorphism (C825T) in exon 10 of the gene encoding the beta3 subunit of heterotrimeric G proteins (GNB3). The T allele is associated with the occurrence of a splice variant, GNB3-s (encoding G beta3-s), in which the nucleotides 498-620 of exon 9 are deleted. This in-frame deletion causes the loss of 41 amino acids and one WD repeat domain of the G beta subunit. By western-blot analysis, G beta3-s appears to be predominantly expressed in cells from individuals carrying the T allele. Significant enhancement of stimulated GTPgammaS binding to Sf9 insect cells expressing G beta3-s together with G alpha(i)2 and G gamma5 indicates that this splice variant is biologically active. Genotype analysis of 427 normotensive and 426 hypertensive subjects suggests a significant association of the T allele with essential hypertension.

Shock wave emission and cavitation bubble expansion after optical breakdown in water with Nd:YAG laser pulses of 30-ps and 6-ns duration is investigated for energies between 50 μJ and 10 mJ which are often used for intraocular laser surgery. Time-resolved photography is applied to measure the position of the shock front and the bubble wall as a function of time. The photographs are used to determine the shock front and bubble wall velocity as well as the shock wave pressure as a function of time or position. Calculations of the bubble formation and shock wave emission are performed using the Gilmore model of cavitation bubble dynamics and the Kirkwood–Bethe hypothesis. The calculations are based on the laser pulse duration, the size of the plasma, and the maximally expanded cavitation bubble, i.e., on easily measurable parameters. They yield the dynamics of the bubble wall, the pressure evolution inside the bubble, and pressure profiles in the surrounding liquid at fixed times after the start of the laser pulse. The results of the calculations agree well with the experimental data. A large percentage of the laser pulse energy (up to 72%) is transformed into the mechanical energy ES and EB of the shock wave and cavitation bubble, whereby the partitioning between ES and EB is approximately equal. 65%–85% of ES is dissipated during the first 10 mm of shock wave propagation. The pressure at the plasma rim ranges from 1300 MPa (50 μJ, 30 ps) to 7150 MPa (10 mJ, 6 ns). The calculated initial shock wave duration has values between 20 and 58 ns, the duration measured 10 mm away from the plasma is between 43 and 148 ns. A formation phase of the shock front occurs after the ns pulses, but not after the ps pulses where the shock front exists already 100 ps after the start of the laser pulse. After shock front formation, the pressure decays approximately proportional to r−2, and at pressure values below 100 MPa proportional to r−1.06. The maximum bubble wall velocity ranges from 390 to 2450 m/s. The calculations of bubble and shock wave dynamics can cover a large parameter range and may thus serve as a tool for the optimization of laser parameters in medical laser applications.

No abstract

NdYAG laser photodisruption with nanosecond (ns) pulses is an established method for intraocular surgery. In order to assess whether an increased precision can be achieved by the use of picosecond (ps) pulses, the plasma size, the shock wave characteristics, and the cavitation bubble expansion after optical breakdown with ps-and ns-laser pulses were investigated by time-resolved photography and acoustic measurements. NdYAG laser pulses with a duration of 30 ps and 6 ns, respectively, were focused into a water-filled glass cuvette. Frequency doubled light from the same laser pulses was optically delayed between 2 ns and 136 ns and used as illumination light source for photography. Since the individual events were well reproducible, the shock wave and bubble wall position could be determined as a function of time. From the slope of these r(t) curves, the shock wave and bubble wall velocities were determined, and the shock wave pressure was calculated from the shock velocity. The plasma size at various laser pulse energies was measured from photographs of the plasma radiation. The breakdown thresholds at 30 ps and 6 ns pulse duration were found to be 15 p J and 200 pJ, respectively. At threshold, ps-plasmas are shorter than ns-plasmas, but at the same pulse energy they are always -2.5 times longer. The initial shock pressures were 17 kbar after ps-pulses with an energy of 50 pJ, and 21 kbar after 1 m J ns-pulses. The pressure amplitude decayed much faster after the ps-pulses. The maximum expansion velocity of the cavitation bubble was 350 m/s after a 50 pJ ps-pulse, but 1,600 m/s after a 1 m J ns-pulse. The side effects of intraocular microsurgery associated with shock wave emission and cavitation bubble expansion can be considerably reduced by the use of ps-pulses, and new applications of photodisruption may become possible. o 1994 WiIey-~iss, Inc.

No abstract

We propose a spatial modulator for terahertz waves based on light induced electron plasma in photo-active semiconductors. A two-dimensional array of computer controlled light is used to create free carries in bulk silicon, which results in a spatial modulation of the transmission at terahertz frequencies. This method not only exhibits a remarkable modulation depth over a broad frequency range but also allows for an optically controlled beam steering of terahertz waves by inducing virtual grating structures. In addition, we analyze the possibility of all-optically controlled terahertz imaging.

Abstract-Recent studies have shown that a polymorphism (C825T) in the gene encoding the G protein ␤3 subunit (GNB3) is associated with hypertension and obesity. We characterized the entire GNB3 gene, which spans 7.5 kb and is composed of 11 exons and 10 introns. Its promoter lacks a TATA box but harbors GC-rich regions. The functional activity of the GNB3 promoter was verified with reporter gene assays that also demonstrated its inducibility by phorbol esters. A novel polymorphism in the promoter region A(Ϫ350)G occurred with frequencies (G allele) of 76%, 97%, and 61% in Africans, Chinese, and Germans, respectively. Reporter gene constructs with either the A or the G allele did not differ with regard to inducement of the reporter protein. A silent nucleotide exchange in the coding region (A657T) occurred with T allele frequencies ranging from 0.5% to 2.4%. Another polymorphism (G814A) results in the replacement of glycine by serine at position 272. In Germans, the A allele occurred at a frequency of 10%. Finally, a C1429T polymorphism in the 3Ј untranslated region of GNB3 was identified that occurred at T allele frequencies of 38%, 17%, and 30% in Africans, Chinese, and Germans, respectively. Haplotype prediction indicated in Germans an almost complete association of GNB3 825T with 1429T, and vice versa. An analysis of these polymorphic loci in nonhuman primates revealed that the ancestral GNB3 gene harbored the (Ϫ350)G, 825C, and 1429C alleles. This is the first complete characterization of the human GNB3 gene and its promoter region, which will enable refined epidemiological and biochemical investigations of GNB3 in hypertension and obesity. (Hypertension. 2000;36:33-41.)

The KATRIN experiment will probe the neutrino mass by measuring the β-electron energy spectrum near the endpoint of tritium β-decay. An integral energy analysis will be performed by an electro-static spectrometer ("Main Spectrometer"), an ultra-high vacuum vessel with a length of 23.2 m, a volume of 1240 m 3 , and a complex inner electrode system with about 120 000 individual parts. The strong magnetic field that guides the β-electrons is provided by super-conducting solenoids at both ends of the spectrometer. Its influence on turbo-molecular pumps and vacuum gauges had to be considered. A system consisting of 6 turbo-molecular pumps and 3 km of non-evaporable getter strips has been deployed and was tested during the commissioning of the spectrometer. In this paper the configuration, the commissioning with bake-out at 300 • C, and the performance of this system are presented in detail. The vacuum system has to maintain a pressure in the 10 −11 mbar range. It is demonstrated that the performance of the system is already close to these stringent functional requirements for the KATRIN experiment, which will start at the end of 2016.