Background: VasantKusumakar Ras (VK Ras) is a traditional Ayurvedic preparation used in the treatment of Type-2 diabetes mellitus. Despite its clinical anti-diabetic claims, no pre-clinical attempts were made to rule out its efficacy as an antidiabetic agent. Objectives: The present study was carried out to find the anti-diabetic effect of VK Ras against a High-Fat Diet (HFD), and low-dose streptozotocin (STZ) induced type 2 diabetes and to explore the mode of action of VK Ras. Materials and Methods: Different doses of VK Ras were administered to diabetic rats for 35 days. The biochemical markers analysis, intestinal glucose uptake, and liver glycogen content were estimated at the end of the study and also vital organs were weighed and subjected to histopathological evaluation. Results: VK Ras treatment reduced blood glucose in a dose-dependent manner. The insulin, HbA1C, HOMA-IR, and lipid profiles were improved in VK Ras-treated animals as compared to diabetic control animals. The relative organ weights were changed in diabetic rats, and treatment with VK Ras corrected the organ weights. Intestinal glucose uptake and liver glycogen content were decreased with treatment. Further, the histopathological analysis of the pancreas and other vital organs had shown that dose-dependent restoration of organ function with VK Ras treatment. Conclusions: VK Ras treatment reduces insulin resistance as well as corrects the lipid, hepatic and renal abnormalities that arise from diabetes, these effects may be mediated by interfering with glucose transport from the gut and insulin release from the β pancreatic cells.
Aim: To evaluate the antidiabetic activity of hydroalcoholic extracts of Ficus gibbosa Blume (leaves and stem bark) in streptozotocin–nicotinamide induced diabetic rats. Materials and methods: The animals were rendered diabetic by single intraperitoneal injections of nicotinamide (195 mg/kg body weight) followed by streptozotocin (65 mg/kg body weight). Induction of experimental diabetes was confirmed by blood glucose analysis. The test extracts were used at 3-dose levels viz., low dose (100 mg/kg body weight), average dose (250 mg/kg body weight and high dose (500 mg/kg body weight). Male Sprague Dawley rats were used in the experiment, each containing 6 animals. Oral glucose tolerance test was carried out 7 days post test extract administration at 3 doses. The diabetic animals were given the test extracts at different doses for a period of 5 weeks and blood sugar was evaluated at weekly intervals. Results: Both leaves and stem bark extracts of Ficus gibbosa Blume significantly reduced the elevated blood glucose levels at 60- and 120-minute postglucose overload. The test extracts showed significant antidiabetic activity against streptozotocin- and nicotinamide-induced diabetes in Sprague Dawley (SD) rats. Conclusion: The extracts of Ficus gibbosa Blume showed significant antidiabetic activity against experimental type 2 diabetes in SD rats.
Vasant Kusumakar Ras is an herbo-mineral formulation known for its anti-diabetic activity. Ayurvedic literature describes Vasant Kusumakar Ras to possess Pramehgana (anti-diabetic), properties. The objective of the current study is to investigate the hypoglycaemic effect of Vasant Kusumakar Ras in fasted normoglycemic and antihyperglycemic effect in oral glucose induced hyperglycemic rats. The male and female Sprague Dawley rats were randomized in to 3 groups based on the bodyweight. One group serves as control which was treated with vehicle and other 2 groups of animals were treated with Vasant Kusumakar Ras 25 mg/kg and Glibenclamide 10 mg/kg respectively. The duration of the drug treatment in hypoglycaemic study was for a period of 7days.The blood glucose levels were analysed in fasted normoglycemic animals on 1st, 3rd&7th day, prior as well as post 01 hr of drug administration. Similarly,the oral glucose tolerance test (OGTT) was carried out on day 8th by challenging the same rats with oral glucose (2 g / kg). The drugs were administered simultaneously the blood glucose was analysed at 0th ,1st, 2nd and 3rdHr of post drug treatment. During the hypoglycaemic study on day 7th, the Vasant Kusumakar Ras treatment signicantly reduced the blood glucose levels (p<0.05). Similarly, during the OGTT the test drug reversed the hyperglycemia induced by oral glucose at 2nd and 3rd Hr intervals. From this study it was concluded that the Ayurvedic formulation Vasant Kusumakar Ras possess signicant hypoglycaemic activity in fasted normoglycemic and antihyperglycemic effect in glucose induced hyperglycemic rats. This may suggest the ethno pharmacological use of Vasant Kusumakar Ras to reduce the hyperglycaemic episodes in diabetic and hyperglycemic non diabetic subjects.
The objective of the present study was to validate a rat model of diabetic dyslipidaemia along with hepatic damage by High fat diet and low dose streptozotocin combination that resembles the natural history of metabolic syndrome in humans. Male and Female Sprague Dawley rats were divided into 2 groups. One group of rats fed with high fat diet and other group was fed with normal pellet diet for a period of 2 weeks followed by streptozotocin administration on 15th day. Body weight of the animals was measured on day 0, 14 and 21, also post 1 week of the streptozotocin administration the blood glucose, serum lipid, liver, kidney markers and insulin levels were measured. The high fat diet fed animals had exhibited a significant increase in body weight post two weeks of HFD feeding, but the body weight was reduced post 1 week of streptozotocin intervention due to metabolic disruption caused by STZ. The HFD group animals exhibited significant increase in blood glucose, serum total cholesterol, triglycerides, LDL, VLDL. Similarly, a significant decrease in HDL and serum insulin levels were observed as compared to the NPD fed animals. Further the HFD group animals had shown significant elevation of the liver function enzymes such as ALT and ALP levels as compared to the NPD fed animals. The present study confirms the experimental induction of diabetic dyslipidaemia along with hepatic damage which serves as an ideal model for metabolic syndrome along with hepatic cirrhosis which would useful for evaluating the therapeutic agents against type 2 diabetes, hyperlipidaemia and hepatic cirrhosis.
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