ABSTRACT Pomegranate (Punica granatum L) is widely used for health. Pomegranate peel is part of the pomegranate fruit which is often discarded and has not been widely used. Pomegranate peel is thought to contain antioxidants. One of the examinations for antioxidants is the 1,1 dipheyl-2-picrylhydrazyl (DPPH) method, by calculating the IC50 value, where the smaller the IC50 value means that the stronger of antioxidant potential. The purpose of this study was to compare the antioxidant activity of the ethanol extract, ethyl acetate fraction, and the active isolate from pomegranate peel. The antioxidant potency was tested using the DPPH, using the UV-Vis spectrophotometer, at 515 nm wavelength. Isolation of isolates was carried out by column chromatography shepadex and TLC with toluene as mobile phase; ethyl acetate : formic acid : methanol, with a ratio of 3:3:0.8:0.2. The purity of the isolate was detected by HPLC, with water-methanol as the mobile phase. The research results showed that 96% ethanol extract, ethyl acetate fraction and active isolate from pomegranate peel have potential as antioxidants with IC50 4.52 ± 0.00, 2.71 ± 0.01, and 0.979 x 10-5 ± 0.00 µg/mL, respectively, the positive control was vitamin C, had an IC50 3.66 ± 0.02 µg/mL. The conclusion of this study is that pomegranate peel isolate has the strongest antioxidant potential, compared to 96% ethanol extract and the ethyl acetate fraction of pomegranate peel, and even vitamin C. ABSTRAK Delima (Punica granatum L) banyak dimanfaatkan untuk kesehatan. Kulit delima merupakan bagian dari delima yang sering kali dibuang dan belum banyak dimanfaatkan. Kulit delima diduga memiliki kandungan antioksidan. Salah satu pemeriksaan antioksidan adalah dengan metode 1,1 dipheyl-2-picrylhydrazyl (DPPH), dengan menghitung nilai IC50, dimana semakin kecil nilai IC50 dapat diartikan bahwa semakin kuat potensi antioksidannya. Tujuan dari penelitian ini adalah untuk membandingkan aktivitas antioksidan dari ektrak etanol, fraksi etil asetat, dan isolat aktif kulit delima. Potensi antioksidan diuji dengan metode DPPH, dengan menggunakan spektrofotometer UV-Vis, pada panjang gelombang 515 nm. Isolasi isolat dilakukan dengan kromatografi kolom shepadex dan KLT dengan fase gerak toluene: etil asetat: asam format: metanol, dengan perbandingan 3:3:0.8:0.2. Kemurnian isolat di deteksi dengan HPLC, dengan fase gerak air-metanol. Hasil peneiltian menunjukan bahwa ekstrak etanol 96%, fraksi etil asetat dan isolat aktif dari kulit delima memiliki potensi sebagai antioksidan, dengan nilai IC50 masing-masing sebesar 4,52 ± 0,00; 2,71 ± 0,01; dan 0,979 x 10-5 ± 0,00 µg/ mL, sebagai kontrol positif vitamin C memiliki nilai IC50 3,66 ± 0,02 µg/ mL. Kesimpulan penelitian ini adalah bahwa isolat kulit delima memiliki potensi antioksidan terkuat, dibandingkan ekstrak etanol 96% dan fraksi etil asetat kulit delima, bahkan juga vitamin C.
<p> </p><p><strong>Pendahuluan</strong> : GKSO (Gerakan Keluarga Sadar Obat) merupakan salah satu program dari Ikatan Apoteker Indonesia (IAI) dalam upaya untuk meningkatkan kesadaran dan pengetahuan masyarakat yang berhubungan dengan penggunaan obat. Hal ini terkait dengan banyaknya permasalahan yang terkait dengan obat yang terjadi di masyarakat. Permasalahan ini bukan hanya menjadi tanggung jawab tenaga kesahatan yang jumlahnya terbatas dan belum mampu untuk memberikan informasi yang cukup mengenai obat, melainkan juga tanggung jawab seluruh komponen masyarakat untuk dapat mewujudkan masyarakat yang cerdas dalam menggunakan obat. Salah satu upaya untuk meningkatkan pengetahuan dan pemahaman masyarakat terkait obat adalah melalui sosialiasi DAGUSIBU ( Dapatkan, Gunakan, Simpan, Buang) obat dengan benar</p><p> </p><p><strong>Metode</strong> : Metode yang digunakan dalam kegiatan pengabdian masyarakat ini yaitu metode pemberdayaan masyarakat partisipatif dengan model <em>Particatory Rural Appraisal</em> (PRA). Dimulai dengan pengenalan masalah yang peserta alami terkait penggunaan obat, selanjutnya diberikan pemaparan materi tentang DAGUSIBU, dan diakhiri dengan diskusi interaktif dan <em>recall memory</em>.</p><p> </p><p><strong>Hasil dan pembahasan</strong> : Kegiatan KIE (Komunikasi, Informasi dan Edukasi) DAGUSIBU kepada sanitarian, dan petugas gizi Kabupaten Karanganyar, berjalan dengan lancar. Para peserta antusias dalam mendengarkan pemaparan materi, dan aktif dalam tanya jawab seputar pengalaman penggunaan obat dalam kehidupan mereka sehari-hari dan bagaimana cara memperoleh, menggunakan, menyimpan dan membuang obat dengan benar.</p><p> </p><p><strong>Kesimpulan</strong> : Kegiatan ini meningkatkan pengetahuan dan pemahaman para sanitarian, dan petugas gizi Kabupaten Karanganyar tentang bagaimana cara pengelolaan obat yang benar melalui program DAGUSIBU. Diharapkan para peserta bisa memanfaatkan pengetahuan yang didapatkan dengan menerapkannya di lingkungan kerja, keluarga dan masyarakat.</p><p> </p><p><strong>Kata kunci : DAGUSIBU; Obat; Karanganyar</strong></p><p><strong>ABSTRACT</strong></p><p><strong>Introduction</strong> : GKSO (Gerakan Keluarga Sadar Obat) is one of the programs of the Indonesian Pharmacists Association (IAI) in an effort to increase public awareness and knowledge related to drugs. This is related to the many problems about drugs that occur in the community. This problem is not only the responsibility of health workers who have not been able to provide sufficient information about drugs, but also the responsibility of all components of society to be able to create an intelligent society in using drugs. One of the efforts to increase public knowledge and understanding regarding drugs is through socialization of DAGUSIBU.</p><p> </p><p><strong>Method</strong>: The method used in this community service activity is a participatory community empowerment method with the Particatory Rural Appraisal (PRA) model. Starting with the introduction of the problems experienced by the participants related to the use of drugs, then the presentation was given about DAGUSIBU, and ended with interactive discussion and memory recall related to the presentation.</p><p> </p><p><strong>Results and discussion</strong>: Communication, information, and education activities about DAGUSIBU for sanitarians, and nutrition workers in Karanganyar Regency, were successfull. The participants were enthusiastic in listening to the presentation, and were active in asking questions about the experience of using drugs in their daily lives and how to obtain, use, store and dispose of drugs properly.</p><p> </p><p><strong>Conclusion</strong>: This activity increases the knowledge and understanding of sanitarians and nutrition workers in Karanganyar Regency on how to properly manage drugs through the DAGUSIBU program. It is hoped that the participants can take advantage of the knowledge by applying it in the work environment, family and community.</p><p> </p><p><strong>Keywords: KIE; DAGUSIBU; Drug; Karanganyar</strong></p>
The number of herbal medicines in Indonesia as cholesterol drugs is still lacking. Guazuma ulmifolia Lamk. is one of the plants suspected of having an anticholesterol effect. The test design is pre and post test with control group design. A total of 30 rats were divided into 5 groups, with each group consisting of 6 rats. Aquadest as a negative control group and simvastatin 0.72mg/200 bw orally is a positive control group. Groups III, IV, and V were treated the test extract with 3 dose levels. A mouse model of hyperlipidemia was performed with Triton-X. Treatment was carried out for 7 days and cholesterol is measured. Liver organs was taken for histopathological examination. The ethanolic extract of the ethyl acetate fraction of Guazuma ulmifolia Lamk was able to reduce total cholesterol in rats induced by a high cholesterol diet. From the histopathological examination, the ethanol extract of the ethyl acetate fraction of Guazuma ulmifolia Lamk improved liver histoPA. The ethanolic extract of the ethyl acetate fraction of Guazuma ulmifolia Lamk has potencies to develop as hypocholesterolemia agent.
Terdapat kesalahan cetak pada versi awal pada artikel https://doi.org/10.23917/reaksi.v4i1.7031.Perbaikan PDF artikel dapat diunduh di laman ini
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