<p>ABSTRAK</p><p>Persilangan kapas yang dilakukan pada tahun 1999 denganmelibatkan 11 varietas kapas sebagai tetua betina dan lima varietas kapassebagai tetua jantan telah menghasilkan 22 set persilangan kapas. Adapuntujuan persilangan tersebut adalah untuk meningkatkan produktivitas danmutu serat varietas kapas nasional Indonesia. Program perbaikan varietasini menggunakan pendekatan seleksi pedigree pada F3, dan dilanjutkandengan seleksi galur pada generasi F5 dan selanjutnya. Pada kegiatanseleksi galur tahun 2004-2005 di Kebun Percobaan Asembagus, dari 22genotipe generasi F4 yang diseleksi dengan kriteria seleksi produktivitas >3 ton kapas berbiji/ha telah dihasilkan 23 galur harapan. Pada generasi F6dilakukan uji daya hasil dari 23 galur tersebut. Dari pengujian daya hasilgalur-galur tersebut pada tahun 2006, telah dihasilkan enam galur harapanyang memiliki tingkat produktivitas secara statistika tidak berbeda denganatau lebih tinggi dari Kanesia 8 dengan panjang serat > 1,16 inch, kekuatanserat > 30,77 g/tex, dan kehalusan serat antara 3,5 – 4,5 mic, yaitu99004/5, 99005/9, 99013/5, 99023/5, 99023/7, dan 99023/8. Galur-galurtersebut selanjutnya akan diuji secara multilokasi untuk menilai stabilitasekspresi genetiknya di beberapa wilayah pengembangan kapas.</p><p>Kata kunci : Gossypium hirsutum, kapas, produktivitas, mutu serat</p><p>ABSTRACT</p><p>Varietal Improvement for Increase of Productivity andQuality of Cotton Fiber Cotton breeding conducted since 1999 involving 11 varieties asfemale parent, and five varieties as male parents has resulted in 22crossing sets. The aim was to improve productivity level as well as fiberquality of national cotton varieties of Indonesia. The breeding approachwas accomplished by pedigree selection on F3 generation, which was thencontinued with line selection from F5 generation. From the line selectionactivity carried out at Asembagus Experimental Station during 2004-2005,out of 22 genotypes selected at the F4 generation based on productivitylevel of > 3 ton seed cotton/ha as selection criteria, 23 promising lines hadbeen selected which were then tested in a yield potential test. From theyield potential test conducted in 2006, six promising lines had beenselected whose productivity levels were statistically not different from orhigher than Kanesia 8 with fiber length of >1.16 inch, fiber strength>30.77 g/tex, and fiber fineness 3.5-4.5 mic. Those promising lines wouldthen be tested in multilocation trials to evaluate the stability of theirgenetic expression in several cotton development areas.</p><p>Key words : Gossypium hirsutum, cotton, productivity, fiber properties</p>
ABSTRAK<br />Produksi benih varietas kapas hibrida dapat ditempuh dengan dua<br />cara, yaitu dengan persilangan manual dan dengan memanfaatkan galur<br />mandul jantan (male-sterile line). Memproduksi benih kapas secara<br />persilangan manual memerlukan tenaga dan biaya yang tinggi, dan biaya<br />tersebut dapat dikurangi dengan menggunakan galur male steril. Penelitian<br />dilaksanakan di Kebun Percobaan Balai Penelitian Tanaman Tembakau<br />dan Serat, di Karangploso, Malang, Jawa Timur, dari bulan April sampai<br />Oktober 2007. Tujuan penelitian ini adalah untuk mengetahui potensi hasil<br />galur-galur mandul jantan kapas untuk memproduksi benih hibrida. Tiga<br />aksesi kapas yaitu KI 487, KI 489, dan KI 494 yang memiliki persentase<br />tanaman mandul jantan masing-masing 60,8%, 57,5%, dan 65% telah<br />digunakan sebagai donor sifat mandul jantan dan telah dilakukan introgresi<br />sifat mandul jantan dari ketiga aksesi tersebut ke varietas komersial<br />Kanesia 7, Kanesia 8, dan Kanesia 9 melalui persilangan pada tahun 2006<br />dan diperoleh 9 set kombinasi persilangan. Pada tahun 2007, evaluasi<br />potensi galur dilakukan terhadap 8 galur F1 mandul jantan, 3 tetua jantan<br />yaitu varietas Kanesia 7, Kanesia 8, dan Kanesia 9, serta satu varietas baru<br />yaitu Kanesia 12 sebagai pembanding yang disusun dalam rancangan acak<br />kelompok yang diulang 3 kali. Plot percobaan berukuran 3 x 10 m 2<br />dengan jarak tanam 100 cm x 25 cm; satu tanaman per lubang. Dosis<br />pupuk yang digunakan adalah 100 kg urea + 100kg ZA + 100kg SP 36 +<br />100kg KCL per ha. Tidak dilakukan pengendalian hama dengan<br />insektisida kimia selama penelitian. Pengamatan yang dilakukan adalah<br />kemandulan benangsari secara visual dan mikroskopis, jumlah buah per<br />tanaman, bobot buah, dan hasil kapas berbiji. Hasil penelitian<br />menunjukkan bahwa pengamatan secara visual dan mikroskopis terhadap<br />struktur bunga menunjukkan bahwa semua individu tanaman dari 8 galur<br />F1 yang diuji adalah mandul jantan. Jumlah buah galur mandul jantan 7 –<br />96% lebih banyak tetapi ukuran buahnya lebih kecil dibandingkan dengan<br />Kanesia. Galur-galur mandul jantan KI 494 x Kanesia 7 dan KI 494 x<br />Kanesia 8 memberikan hasil kapas berbiji paling tinggi masing-masing<br />2.609kg dan 2.153kg per hektar dibandingkan dengan galur-galur lain,<br />atau sebesar 94 % dan 95% dibandingkan dengan Kanesia 7 dan Kanesia<br />8. Persilangan alami galur-galur tersebut bervariasi sebesar 51 – 95%.<br />Kata kunci : Kapas, Gossypium hirsutum. L., mandul jantan, benih hibrida<br />ABSTRACT<br />Cotton yield potential of F1 male sterile lines under<br />natural crossing<br />Cotton hybrid seed production can be done by manual crossing and<br />by using male steril line methods. The manual crossing technique is<br />however labor dan cost intensive, and the cost can only be reduced by<br />using male sterile lines. The experiment was conducted in Karangploso<br />Experimental Station of Indonesian Tobacco and Fiber Crops Research<br />Institute (IToFCRI), Malang, East Java, from April to Oktober 2007<br />aiming to evaluate the yield potential of cotton male sterile lines. Three<br />cotton accessions e.i KI 487, KI 489, and KI 494 which have male sterility<br />percentage of 60.8%, 57.5%, and 65%, respectively, were used as donor<br />for male sterility and were then introgressed to three commercial cotton<br />variety, Kanesia 7, Kanesia 8, and Kanesia 9 through manual crossing, and<br />that resulted in nine sets of crossing combinations. In 2007, yield potential<br />were studied including 8 F1 male sterile lines, 3 male parent lines (Kanesia<br />7, Kanesia 8, and Kanesia 9), and one new cotton variety, Kanesia 12, as<br />control in a randomized block design with 3 replications. Plot size was 3 x<br />10 m 2 with 100 cm x 25 cm plant spacing; one plant per hill. Fertilizer<br />dosage was 100kg urea + 100kg ZA + 100kg SP 36 + 100kg KCl per ha.<br />Chemical insecticide was not used for insect protection during the<br />research. Parameters observed were plants male sterility, number of bolls<br />per plant, boll weight, and seed cotton yield. The experimental result<br />showed that both visual and microscopic observation of male sterility on<br />individual plants confirmed that the eight F1 lines tested were male sterile.<br />Number of bolls per plant of male sterile lines were 7 – 96% higher than<br />that of Kanesia’s, but boll size was smaller. Lines KI 494 x Kanesia 7 and<br />KI 494 x Kanesia 8 produced highest cotton seed yield of 2609 kg and<br />2153 kg per hectar, respectively, which were 94% and 95% of that of their<br />male parents, Kanesia 7 and Kanesia 8, respectively. Natural crossing of<br />those lines varied around 51 – 95%.<br />Key words : Cotton, Gossypium hirsutum. L., male sterile, hybrid seed
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