Nyamplung (Calophyllum inophyllum L.) is one of the most potential plants for biodiesel feedstock because of its high oil content. Mechanical extraction using screw press is one method to get oil from nyamplung seed. Result of the extraction is affected by the seed's condition, such as moisture content and particle size. The paper presents experimental results that estimate the vegetable oil production potential of C. inophyllum. The results show the effect of C. inophyllum seed moisture content and particle size on oil yield, andthe characteristics of C. inophyllum oil.The seed moisture contents used in this experiment are 0%, 1.2%, and 20%, whereas the average seed particle size used are 0.81, 2.90, and 8.60 mm. The C. inophyllum fruits were obtained from Cipatujah Sub-district, Tasikmalaya Regency. The methods used include fruit and seed preparation, seed moisture content and particle size conditioning, mechanical extraction, oil characteristics analysis, and C. inophyllum oil production potential calculation. The optimum seed moisture content to obtain high oil yield is 1.2% which yields 33.39%oil, while the optimum seed particle size to obtain high oil yield is 8.60 mm which yields 33.46% oil. The bigger the particle size will affect on higher oil yield. From this research, it can be concluded that the trees in Cipatujahhave potential to produce C. inophyllum oil up to 5.13 L/tree/year. C. inophyllum oil yield is effected by seed moisture content and particle size, and it has characteristics that support its utilization as biodiesel feedstock.

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A procedure was developed to induce shoot organogenesis of teak (Tectona grandis L.f.) callus cultures. Calli were induced from internodal segments on woody plant medium containing 1.0 µM thidiazuron in combination with 0.01 µM indole butyric acid. Results showed that compact callus, with white to light yellow nodular structures on its surface, were produced in four weeks. The most regenerative callus was noted as the compact callus with light yellow or light green color. Shoot organogenesis was induced on medium containing 10.0 µM benzyl adenine in combination with 1.0 µM gibberellic acid. Adventitious shoots proliferated from the surface of regenerative compact callus after three subcultures. Regenerated shoots were propagated on shoot multiplication medium with the addition of 10.0 µM BA. Most elongated shoots were rooted in a soil-sand mixture medium (1:1), and were established under greenhouse conditions.

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Caffeine and chlorogenic acid (CGA) are two compounds that play a role in determining the quality of coffee. The amount of the two compounds may vary depending on the environment where they are grown. This study aimed to determine the correlation between the local microclimatic condition and the concentration of caffeine and CGA in green and roasted beans of Robusta coffee from six different cultivation areas in West Java, Indonesia (i.e., Ciamis, Tasikmalaya, Sumedang, Kuningan, Cianjur, and Bogor). Samples of green beans and roasted beans were extracted with 70% methanol for caffeine analysis and ethyl acetate for CGA analysis. Caffeine and CGA were analyzed by UV-HPLC using a C18 shimpack gist shimadzu column, with an isocratic elution of methanol:water (1:1) at a 1 mL/min flow rate. Detection was performed at  λ272 nm and λ324 nm for caffeine and chlorogenic acid, respectively. Principal component analysis (PCA) was used to evaluate the correlation between microclimate with caffeine and chlorogenic acid. Results indicated that the concentration of caffeine ranged from 7.67 to 16.52% and 10.79 to 15.56% in the green and roasted bean coffee, respectively. The concentration of CGA ranged from 0.74 to 3.03% and 0.25 to 0.77% in the green and roasted bean coffee, respectively. Based on PCA analysis, the most influential microclimate on the caffeine concentration were the humidity, temperature, and altitude, with the total variance of PC1 and PC2 of  76.3%. However, there was no positive correlation between the measured microclimate and the CGA concentration. In conclusion, Robusta coffee's caffeine content is positively affected by the microclimatic condition (i.e., humidity, temperature, and altitude).

Musa acuminata L. growth has influenced by environment. Environmental stresses are caused by biotic factors such as fungi, bacteria and herbivore and abiotic factors such as temperature, water, light and salinity. Salinity stresses cause a decrease in the production of banana. The purpose of the research was to validity and confirmation 4-Coumarate-CoA ligase Like5 (4CLL5) gene with 18S reference genes as a control genes on planlets of banana with sodium chloride (NaCl) application. 4-Caomarate-CoA-ligase Like5 is an enzyme key in phenyl-propanoid metabolism like lignin and flavonoid that essential for plant defense on abiotic factor like salinity is coded by 4CLL5 on Musa acuminate L. Salinity is abiotic factors which affect the growth of banana plant. Gene expression 4CLL5 on banana by using real-time quantitative polymerase chain reaction. Research using shoot banana in vitro culture with any concentration of sodium chloride (NaCl) were 0.00, 0.05, 0.10, 0.15 and 0.20 M. In addition to, in this research was using 4CLL5 as a primers. The expression of genes analysis was using real-time quantitative polymerase chain reaction. The results showed that 4CLL5 gene was expressed up regulated on NaCl 0.05 and 0.1 M but down regulated in 0.15 and 0.20 M.

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