In our study interobserver reproducibility of Gleason scores among general pathologists was at lower level and it highlights the need to improve the observer reproducibility by continuous educational sessions and taking second opinion in cases where grade could significantly influence management.

Aims and Objectives:1. To find out the incidence of EDTA dependent pseudothrombocytopenia(PTCP) 2. To confirm EDTA dependent pseudothrombocytopaenia(PTCP) by counting the platelets using anticoagulants other than EDTA (citrate and heparin) Introduction: Pseudothrombocytopenia (PTCP) meaning there by a spuriously low platelet count. EDTA induced clumping of platelets is one of the important cause of pseudothrombocytopaenia. Materials and MethodInclusion criteria: All 3000 blood samples received during study period in hematology laboratory of BVDU Medical college sangli for complete blood count (CBC) or platelet count Exclusion criteria: 1. Patients with known cause of thrombocytopenia were excluded. 2. Thrombocytopenia due to technical errors. Result: The incidence of EDTA Dependent Pseudothrombocytopenia was 0.13%. Conclusion:The incidence of EDTA dependent Pseudothrombocytopenia is quite low. Every sample with a low platelet count with EDTA as anticoagulant and/or presence of platelet clumps on the peripheral blood smear, and after ruling out other causes of pseudothrombocytopenia, must be collected again in a vaccutainer containing different anticoagulant (heparin and citrate). If the platelet count rises therein, it should be considered as a case of EDTA dependent Pseudothrombocytopenia.

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