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The genus Artemisia includes the biggest genus of Asteraceae has many healthful uses in human diseases. The present study was conducted to eva-luate in vitro antioxidant and antibacterial activities of field wormwood, Artemisia campestris L. growing wild in the easthern of Algeria. The aerial parts of the plant were dried at shade and extracted with acetone, ethyl acetate, methanol and water. Total phenolic, flavonoid, flavanol and flavone, tannin and anthocyanin contents of different extracts from the whole plant were determined. The obtained results indicated that the ethyl acetate extract possessed high phenolic, flavonoid, flavanol and flavone contents and exhibited good antioxidant activity by DPPH, FRAP, ß- carotene bleaching methods. However, acetone extract showed strong antiradical property against H2O2 and high tannin content. These findings provide evidence that the polyphenolic extract of A. campestris L. is a natural source of antioxidant against oxidative damage. A. campestris extracts were also tested in vitro for its antimicrobial potential against three strains of pathogenic bacteria, using the disk diffusion method. The extracts showed the best antibacterial effect for all microbial strains tested even those resistant to some antibiotics. Fractionation and characterization of A. campestris active components may enhance the pharmaceutical industry of new drugs against pathogenic bacteria.

The present work is carried out to evaluate biological activities of tubers extracts of Bunium mauritanicum. The methanol extract yield had higher (7.81%) tha n that of the aqueous extract (6.79%). The quantitative analysis of total phenols and flavonoid revealed that the highest concentration was recorded for the methanoic fraction with 89,442 ± 5,951μg EAG mg and 4.031 ± 0.141 μg EQ mg of extract respectively. In addition, the aqueous extract of tubers represents the most important antioxidant activity with an IC 50 of 0.14 mg ml against 2,2 diphenyl 1 picryl hydrazyl (DPPH Wherea s, the two extracts of the tubers have low reducing capacities compared to standard with EC 50 equal to 0.048 mg/ ml for the methanolic extract, 0.018 mg ml for t he aqueous extract and 0.009 mg/ ml for ascorbic acid. The methano l ic and aqueous extracts of Bunium mauritanicum reacted positively at least on one of the bacterial strains studied.