As an alternative to seed propagation, an efficient micropropgation of Asplenium nidus and subsequent rooting were developed as an option for in vitro conversation purpose. Spores were used as an explant. Different growth hormones were used to study the response of fern to the different stages of propagation in vitro, consequently, to determine the best hormone concentration that give up best initiation and proliferation for prothalli, differentiation and rooting. Results show that all studied treatments increased the mean of pothalli length, width and height and the highest values (2.64 cm, 2.44 cm and 2.81 cm for prothalli length, width and height respectively) were observed when MS medium were supplemented with 3.0 mg/L KIN (kinetin) + 0.1 mg/L NAA (naphthalene acetic acid), meanwhile, the lowest values (0.84 cm, 0.59 am and 0.96 cm for prothalli length, width and height respectively) were observed in control. A maximum of 5.28 shoot per microshoot were obtained on Murashige and Skoog agar medium supplemented with 2.0 mg/L 6-benzyl amino purine (BAP) and 0.1 mg/L of IBA (indole-3-butyric-acid). The highest leaves number (4.56) and stem height (5.50 cm) were observed when BAP and IBA were added respectively at 2.0 mg/L and 0.3 mg/L. Maximum root number (8.91 root/ex-plant) was obtained from media contained 1.0 mg/L naphthalene acetic acid (NAA). Meanwhile, maximum roots length (6.23 cm) and leaves number (5.39) were observed when NAA was added at 0.5 mg/L. In vitro propagation of fern can be applied to produce those species of ferns that are hard to propagate conventionally for the benefit of the ornamental industry.
R e p le 18 octobre 1990 HADDAD, S., et COUDRET, A. 1991. Effets de I'adjonction de KC1 ou de CaCI, sur la tolerance au NaCl chez deux cultivars de triticale (Clercal et Beagle). Can. J. Bot. 69 : 21 13-2121. Les effets de la saliniti sur la croissance ainsi que les relations hydriques et ioniques (K' et Na') sont CtudiCs chez deux cultivars de triticale (Clercal et Beagle), aprks 21 jours de culture en conditions contr6lees sur des solutions nutritives additionnkes ou non de NaCl 150 mM et sur des solutions nutritives avec 150 mM + KC1 20 mM ou NaCl 150 mM +CaCI, 20 mM. L'augmentation de la concentration de NaCl dans le milieu de culture affecte fortement la croissance en provoquant un deficit hydrique dO a la chute des potentiels hydriques (9) et osmotiques (T) et entraine une accumulation de Na' et une diminution de K' au niveau des parties aeriennes des triticales. L'addition de KC1 ou de CaCl, sur les milieux avec NaCl ameliore la tolerance des plantes au NaCl : stimulation de la croissance de plus de 30%, amelioration de l'hydratation des tissus, maintien d'une alimentation normale en K' se traduisant par I'augmentation du rapport de sClectivitC K'INa' dans les parties aeriennes. Les deux cultivars ne presentent pas le m&me degrC de tolerance sur ces milieux. Le ( Clercal , semble davantage protege en prtsence de KC1 ou de CaClz dans les milieux avec NaCl que le Beagle ,. I1 apparait par ailleurs, en presence de KC1 ou de CaCl,, que I'amClioration de l'alimentation en K' est suptrieure chez le c Clercal , que chez le < Beagle ,. I1 se pourrait donc que le c Beagle, ait besoin de quantites moindres de K' que le ( Clercal ,, pour assurer son bon fonctionnement cellulaire. La maintien d'un bon rapport de s6lectivitC K'INa' semble un critkre determinant pour la tolerance des triticales a la salinite.Mots clPs : croissance, relations hydriques, ajustement osmotique, sClectivitC K+-Naf, c Clercal ,, Beagle ,, Triticale; tolerance au NaCI, KCI, et CaCI,.HADDAD, S., and COUDRET, A. 1991. Effets de l'adjonction de KC1 ou de CaCI, sur la tolerance au NaCl chez deux cultivars de triticale (Clercal et Beagle). Can. J. Bot. 69: 21 13-2121. The effects of salinity on growth along with water and ion relations were studied on two cultivars of triticale (Clercal and Beagle) after 21 days of growth under controlled conditions in nutrient solutions with or without 150 mM NaCI, and on nutrient solutions with 150 mM NaCl + 20 mM KC1 or 150 mM NaCl + 20 mM CaC1,. Growth was strongly affected by an increased NaCl concentration in the culture medium, causing a water deficit linked to a drop in water ($) and osmotic (T) potentials, followed by an accumulation of Na' and a reduction of K' in the aboveground part of the triticales. Addition of KC1 or CaCl, to NaCl-containing media improved NaCl tolerance of the plant: growth increased more than 30%, hydration of tissues improved, and K' uptake was normal, as seen by an increased K+/Na+ selectivity ratio in the aboveground parts. The two cultivars did not show the same degree of toler...
The experiment was conducted in Hama governorate (Bammraa village) in Syria during the spring and summer seasons 2013-2014 to study the effect of photoperiod on some production indicators (Date of flowering, Number of flower clusters, Number of flowers, Diameter of flower cluster and length stem). Floral induction of three Hydrangea macrophylla. Ser cultivars ("Mme. Emile Mouillè re", "Nikko Blue", "Pia" was evaluated to determine the flowering. Plants were tested under either natural day length (ND) or 24-hours-extended day (ED) or 8-hours short day (SD). Floral induction of H. macrophylla occurred under all treatments but was more rapid under ED. Number of flower clusters, flower numbers and diameter of flower cluster were significantly different for cultivars and photoperiods. Floral initiation and development were observed among photoperiods. Results indicated that ED was the best in all production indicators (Number of flower clusters, Number of flowers, Diameter of flower cluster and length stem) in the cultivars with significant differences. In addition, the results showed that the cultivar (Nikko blue) was more responsive in all production indicators but, the amount of anthocyanin was more in deep pink sepals than blue sepals.
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