The scale morphology of nine Mullidae taxa consisting of three genera and nine species from the Persian Gulf and the Gulf of Oman was described and compared using light and scanning electron microscopy from four different body regions. The general scale type in the studied mullid species was ctenoid except in the head region of Mulloidichthys vanicolensis and Parupeneus margaritatus, which had cycloid scales. The scales demonstrated a large focus with the central or centro‐posterior position. The large variations of scale morphology were observed for the scales from different body regions of a single species. The shape of focus was in five types with the round type being the most common. In the scales of most examined species, there were 5–6 radii present in the anterior field. No radii exist in the lateral and posterior fields, and the radii orientation was parallel in all the studied species. The rostral margin of scales represented five types among the studied species; waved (M. vanicolensis), smooth in (P. rubescens), dentate (Upeneus doriae), scalloped (U. vittatus), and fluted (U. tragula). The lepidont shape varied among the species from blunt to flat, pointed, tiny, sharp, pointed, triangle, short, and long. The results also showed that the relative scale size has a desirable contribution to separate the examined genera. The scale morphological‐based tree was largely consistent with the known systematics of the studied fishes. It was concluded that variation of scale characters has probably taxonomic and even phylogenetic information in both species and genus levels in the studied mullid fishes. However, variation of scale morphology between body regions, as shown in this study, suggests that scale characters should be used cautiously for taxonomic studies of these fishes.
Scale and otolith morphology and morphometry of Indian oil sardine Sardinella longiceps (Clupeidae) were investigated and described using light and scanning electron microscopy from eight different body regions for scales and the right and left otoliths. Scales of the Indian oil sardine show general characteristics of the other studied clupeids sand that are easily distinguishable from other fish groups, by having striae in the posterior field. The studied cycloid scales of S. longiceps were classified into three types based on the overall shape including circular (e.g. true circular and cordate), pentagonal and quadrilateral in the different body regions. The circular shape was the most common shape (87.5%), while the quadrilateral and pentagonal forms constituted 6.25% each. The results also showed that the relative scale size (J-index) plays a desirable contribution in separating the examined populations. The results showed that the mean (or relative) scale size for all the eight regions in the Oman Sea population is larger than the Arabian Sea population. Also, another scale variable, the scale shape index (Si index), demonstrated variation (a mean of 0.86 to 1.1) in different regions of both populations from the Oman and Arabian Seas. Interestingly, here, we found that scale characters of S. longiceps not only differ from its other congeneric species, but also differ in the populations from both sides of the Oman Sea (Iran and Oman) and the Arabian Sea. It shows a positive signal for the presence of different taxonomic and management unit in the Oman and Arabian Seas. The idea should be approved by using integrated molecular and morphological traits. The otolith morphology of S. longiceps from the Oman and Arabian Seas was more conservative than the scales, which can be due to its function actin primarily as a balance organ and also enhancing hearing. The overall shape of S. longiceps otolith was lanceolate, with an elongated morphology and a well-developed rostrum, an ostial sulcus acusticus that opens to the anterior/ dorsal margin. These morphological characters are also found in the Iranian population of S. longiceps. However, otolith displayed variation in biometric parameters among two populations and left and right otoliths and the RRL parameter were important characters to discriminate
Secondary sexual dimorphism is an interesting and stupendous subject of research study due to the fact that it is a result of sexual selection, mostly attributed to either female mate choice or male–male competition. This study aims to investigate the microscopic characterization of scales and fin rays in several aphaniids (Cyprinodontiformes: Aphaniidae) to find out the possibility of using these structures as secondary sexual dimorphism traits and discuss their taxonomic and evolutionary significance. Herein, the results reveal that (i) male individuals of aphaniid species of the genera Aphanius, Aphaniops and Paraphanius present contact organs in the forms of ctenus‐like structure (in posterior margin of the scales) and spicule‐like structure (in anal fin rays) that show sexual dimorphism of scales and fins; (ii) number, position, size and shape of contact organs provide taxonomic signal to distinguish members of the genus Aphaniops from two other genera; (iii) females of Aphaniops ginaonis also represent contact organs in scales but fewer than males and (iv) as the sexual dimorphism results from different sexual selection pressures acting on males and females, it provides evolutionary signals.
The optical light microscopy and scanning electron microscopy techniques have proven to play a key and noteworthy role in the advancement of morphological studies in general, and in investigating fish scale morphology in particular. These techniques have illustrated several hidden architectural structures in scales that contribute effectively to fish identification and classification. The scale morphological and topological characters such as type, size, shape, lateral surface, focus position, circuli appearance, radii type, lepidonts, and posterior and anterior margin shapes were obtained using macro‐ and microscopic analysis in six body regions for three size classes of Garra sharq, a cyprinid endemic fish of the Arabian Peninsula. The general scale type in the studied G. sharq species was a basal elasmoid cycloid and a sectioned type. As a protective structure, the scales display several specific characteristics including firm attachment to the fish body, overlapping, and thin structure with a high surface area and high strength. These characteristics improve scale resistance to penetration, increase protection against mechanical injury and microbial infection, enhance scale flexibility, reduce fish weight (reduce friction drag), and increase scale transparency. The scales demonstrate plasticity in focus shape, size, and position in the six fish body parts and fish size groups. The examined scales displayed narrow or wide grooves (radii) in three types including primary, secondary, and tertiary present in all four scale fields (anterior, posterior, and laterals), thus a tetra‐sectioned type that is almost specific to the genus Garra. This characteristic also increases scale flexibility. The rostral margin of scales was characterized by the presence of waved and striate types. The lepidont shape and size varied being blunt, flat, pointed, tiny, sharp, short, and long. Some of these scale characters and their morphologies could be used as an alternative tool for identification, classification, and phylogenetic interpretation among the different freshwater fish species and genera.
The complex architecture of fish scale has been investigated for several decades to show an existing link between scale morphology/microstructure and systematic/ evolutionary relationships of fishes. In the present study, the surface architecture structure associated with the scales of a hill stream cyprinid fish Garra shamal restricted to the Hajar Mountains of the Arabian Peninsula was examined by optical light and scanning electron microscopy; (i) to describe the whole morphology, architectural design and hidden microstructures of scales in an endemic fish of the Arabian Peninsula, G. shamal, (ii) to investigate the phenotypic plasticity of scales in six selected regions of the fish body, (iii) to show an ontogenetic pattern of scale morphology in different size classes, and (iv) to study morphometric parameters for scales, and determine the most valuable indices for differentiating the scales of different body regions and the different size groups of studied species. For this purpose, scales were removed from six body regions of three size classes. The scale of G. shamal, as an ossified platelet and hard structure covering the outer part of fish in different body parts and size groups, demonstrated numerous hidden morphological and structural characteristics, and sculptural design, for example, type, shape, and size in general term, and circuli (thickness, arrangement, and relative spacing of circuli around the focus), radii (number, shape, its origin from focus), lepidonts (the texture, shape, size, orientation, and type of its attachment), and granules (shape, size, number, and configurations) in a specific term, that can contribute efficiently in fish identification and classification at the genus and species levels. Position of focus, presence of radii in all four fields of scales (anterior, posterior, and laterals) and thus tetra-sectioned type, presence of specific tubercle arrangement on the posterior region, and some scale indices can be used as a taxonomic tool in Garra identification.Moreover, scales in G. shamal demonstrated plasticity in different fish size classes, and the origin of scales on the fish body, revealing a kind of ontogenetic variation, and the importance of key scales (scales below the dorsal fin) in the lepidological studies. The quantitative and qualitative traits described here based on the optical light microscopy and electron scanning microscopy can be implemented along with
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