The purpose of this study was to determine the effect of colchicine on chromosome duplication and to determine the best colchicine concentration and incubation time for haploid eggplant chromosome duplication results from anther culture. Laboratory experiments have been carried out at PT. East West Seed Indonesia, Benteng Village, Campaka District, Purwakarta from March to June 2020. With an altitude of 69 m above sea level. The environmental design used in this study was a completely randomized design consisting of 7 treatments and repeated 4 times. Colchicine treatment design A = Control, B = colchicine 0.05% 24 hours incubation, C = colchicine 0.05% 48 hours incubation, D = colchicine 0.1% 24 hours incubation, E = colchicine 0.1% 48 hours incubation, F = kolkisisn 0.15% incubation 24 hours, G = colchicine 0.15% incubation 48 hours. The responses observed were contamination, callous plantlets, rooted plantlets, live plantlets, haploid plantlets, double haploid plantlets, triploid plantets and unreadable ploidy check results. The results showed that the colchysis concentration and incubation time had an effect on the duplication of haploid eggplant plantlet chromosomes resulting from anther culture. And giving colchicine at a concentration of 0.05% with an incubation time of 48 hours and a colchicine concentration of 0.1% with an incubation time of 48 hours had a significant effect on the double haploid plantet of 23.44%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.