Here we describe the potential uptake and assimilation pathways for the essential nutrients C, N, P, and S in the acidophilic iron oxidiser Ferrovum strain JA12, a member of a novel genus among the Betaproteobacteria. Comparative genomics proved to be a powerful approach to give first insights into the biochemical potential of this novel genus and to understand the reasons for the dominating abundance of Ferrovum spp. in a pilot plant to remediate acid mine drainage.
a b s t r a c tHxk2 is the predominant hexokinase of Saccharomyces cerevisiae during growth on glucose. In addition to its role in glycolysis, the enzyme is involved in glucose sensing and regulation of gene expression. Glucose limitation causes the phosphorylation of Hxk2 at serine-15 which affects the nucleocytoplasmic distribution and dimer stability of the enzyme. In order to identify the responsible kinase, we screened selected protein kinase single-gene deletion mutants by high resolution clear native PAGE. Deletion of YMR291W/TDA1 resulted in the absence of the Hxk2 phosphomonomer, indicating an indispensable role of the corresponding protein in Hxk2 phosphorylation.
Ferrovum is a recently found new genus of acidophilic iron-oxidizing bacteria.Ferrovumspp. dominate the microbial community of a pilot plant for biological mine-water treatment and together with someGallionellarelatives appear to be key players of the process. Isolation ofFerrovumstrains should greatly be facilitated by a new APPW medium. Sequencing of the genome ofFerrovumsp. JA12 so far did not point to any alternative electron donors and also did not reveal genes for nitrogen fixation.
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