The method of voltammetric determination of nitroimidazoles in honey is developed. This method is based on the reduction of nitroimidazoles nitro group on the mercury drop indicator electrode. The obtained values of LODs for nitroimidazoles are as follows: 1.5 × 10 −7 M, 8.6 × 10 −8 M and 2.0 × 10 −7 M for metronidazole, dimetridazole, and ipronidazole, and the linear correlation coefficients are 0.99974, 0.99987 and 0.99849, respectively, which tend towards to 1. The minimum amount of metronidazole that can be detected by means of our method, using simple dissolving of honey sample in water is 300 µg of metronidazole per 1 kg of honey. The developed voltammetric technique's accuracy was confirmed by the "added-found" method and by HPLC-MS/MS analysis. The calculated amount of metronidazole in the tested model solution is in good agreement with the amount, introduced into honey. The calculated values of t exp are 0.52, 0.48 and 0.43 for three different concentrations. Significant advantages of the method are effortless sample preparation, fast procedure of analysis, the possibility of miniaturization, low cost, not necessary use of organic solvents.
The voltammetric behaviour of coccidiostat robenidine was investigated. The outcomes have revealed that reduction of robenidine causes an appearance of one diffusion‐controlled cathodic peak on a static mercury drop electrode (SMDE) and a silver solid amalgam electrode (p‐AgSAE). The influence of pH, a supporting electrolyte, a scan rate and accumulation parameters was evaluated. The mechanism of robenidine reduction involving two electrons and one proton was studied for the first time. The calibration curves with the wide linear concentration ranges (more than two concentration orders) were obtained under optimized experimental conditions and operating parameters. The reduction current linearly increases when increasing the concentration of coccidiostat. The methods of voltammetric determination of robenidine in feed and meat have been developed. The limits of quantitation of the methods of robenidine determination in feed are 9.5 mg/kg and 2.5 mg/kg on SMDE and p‐AgSAE, respectively. Calculated recoveries ranges of robenidine were between 88.8 % and 101.5 %.
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