Two bacterial strains designated as W3-2-3T and HKS04T were isolated from mineral water and a soil sample, respectively, in the Republic of Korea. The 16S rRNA genes of the two strains shared a sequence similarity of 93.5 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains W3-2-3T and HKS04T formed a distinct lineage within the genus
Nocardioides
of the family
Nocardioidaceae
(order
Propionibacteriales
). The closely related species of strain W3-2-3T were
Nocardioides albidus
(98.9 %),
Nocardioides caeni
(98.8 %),
Nocardioides kongjuensis
(98.6 %),
Nocardioides aromaticivorans
(98.5 %),
Nocardioides nitrophenolicus
(98.4 %),
Nocardioides flava
(98.2 %) and
Nocardioides ginsengisoli
(98.1 %). The closest species of strain HKS04T was
Nocardioides halotolerans
(98.7 %). The genome sizes of strains W3-2-3T and HKS04T were 4741198 and 5 120341 bp, respectively. The genomic DNA G+C contents of strains W3-2-3T and HKS04T were 73.3 and 72.1 mol%, respectively. The main fatty acids of strain W3-2-3T were C17:1
ω6c and iso-C16:0 and those of strain HKS04T were iso-C16:0 and iso-C16:0 H. The main polar lipids of both strains were diphosphatidylglycerol and phosphatidylglycerol and the predominant respiratory quinone was MK-8(H4), supporting the affiliation of these strains with the genus
Nocardioides
. Based on the results of biochemical, chemotaxonomic and phylogenetic analyses, two novel species, Nocardioides convexus W3-2-3T (KACC 21211T=LMG 31251T) and Nocardioides anomalus HKS04T (KACC 18879T=LMG 31249T), are proposed.
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