Introduction Growing evidence suggest that cycle threshold (CT)-value of reverse transcription polymerase chain reaction (RT-PCR) is correlated with transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and some kits set different CT-value cut-off. This report presents the discordant results of two widely used RT-PCR kits in Indonesia due to different CT-value cut-offs and highlights its potential consequence in SARS-CoV-2 containment. Methods Nasopharyngeal swab samples with SARS-CoV-2 negative with a RT-PCR kit (manufacture pre-set CT-value cut-off was 35 amplification cycles) were retested with another RT-PCR kit with a higher pre-set CT-value of 40 amplification cycles. All procedures were performed according to the manufacturer protocols. Results In total, 30 samples with SARS-CoV-2 negative for the first kit were retested. We found that 25 out of 33 samples (75.5%) were positive using the second RT-PCR kit that had a higher manufacture pre-set CT-value cut-off. In addition, among 500 RT-PCR tests using the first RT-PCR kit, 103 of them (20.6%) were categorized as inconclusive results based on the second manufacturer’ guideline. Discussion and conclusion Our data suggest the possibility of discordant results of SARS-CoV-2 detection due to different pre-set cut-offs by the companies. As consequence, this could leave a fraction of individuals who were misclassified that could act as source of virus transmission within community.
Aplikasi Tangerang Live merupakan aplikasi berbasis android yang dikenalkan oleh Pemerintan Kota Tangerang untuk membantu masyarakat Tangerang mengefisensi dan mengefektifkan proses administrasi yang mungkin dibutuhkan oleh masyarakat Tangerang. Penelitian ini dilakukan dengan tujuan untuk mengukur sebuah model kualitas layanan jasa atas sebuah aplikasi yang dikeluarkan oleh Pemerintah Kota Tangerang dan faktor penerimaan teknologi yang baru ini berdasarkan persepsi pengguna dan melihat pengaruh kualitas layanan aplikasi tersebut dengan pendekatan pada teori Skala SERVQUAL dalam kaitannya dengan faktor meningkatkan kualitas jasa. Uji hubungan antara variabel kualitas terhadap keputusan penggunaan dilakukan dengan analisis regresi linear berganda. mengambil sampel obyek penelitian pada mahasiswa program studi manajemen Di Universitas Buddhi Dharma sejumlah 68 responden yang memenuhi syarat untuk diteliti lebih lanjut dengan alat uji statistik SPSS. Hasil penelitian menjelaskan bahwa rata-rata nilai kualitas adalah sebagai berikut : pertama, kualitas layanan situs pada aplikasi memiliki dampak yang cukup signifikan terhadap keputusan penggunaan aplikasi, kedua, layanan dasar situs pada aplikasi memiliki dampak cukup signifikan terhadap keputusan penggunaan aplikasi yang berimplikasi pada peningkatan kualitas layanan jasa itu sendiri. Hal ini sejalan dengan hasil dari metode analisis yang digunakan dalam regresi linear sederhana dengan hasil variabel X1 terhadap Y adalah Y = 20,349 + 0,477 X1 lalu untuk hasil variabel X2 terhadap Y adalah Y = 18,699 + 0,535 X2 dan regresi linear berganda dengan hasil variabel X1, X2 terhadap Y adalah Y = 14,036 + 0,373 X1 + 0,272 X2. Selain itu besaran pengaruh juga didapat dari hasil metode analisis koefisien determinasi, hasilnya X1 memiliki pengaruh kontribusi terhadap Y sebesar 30,8%, X2 memiliki pengaruh kontribusi terhadap Y sebesar 20,9%, X1,X2 memiliki pengaruh kontribusi terhadap Y sebesar 34,7%, hal ini menandakan bahwa studi ini memiliki kontribusi yang cukup signifikan terhadap kaitannya dengan peningkatan kualitas jasa.
This research is motivated by the phenomenon of the Covid-19 Pandemic which has almost hit all nations in the world and has caused a domino effect on the joints of human life itself. Various fields experience tidal effects, including one of them is the economic sector. To deal with uncertain situations and ups and downs like today that can make a country enter the trough of an economic crisis, it is necessary to have support from the central government to the regions to jointly generate the economy. Therefore, this study aims to analyze the factors that influence the interest of the youth community of Gelam Jaya Village to contribute to developing tilapia fish farming which aims to increase the original income of the environment so as to be able to make choices for the community to continue to get income distribution. The problem in this research is the low interest of the youth community to take part in creative economic efforts for the surrounding environment. In this study, a research model was proposed to analyze the interest of young people in actively contributing to tilapia cultivation, where from the location side, Gelam Jaya Village made it possible to develop collaboration with related agencies in terms of generating the creative economy. This study examines the factors that can be obtained when interviewing the village regarding the youth's interest in contributing to the creative economy. This study uses answers from 169 respondents who were sampled. The data analysis tool used is factor analysis in the SPSS v.18 program. The results of data analysis show that the Situational Involvement (X1) factor of 0.766 is one of the most dominating factors for respondents in actively contributing to developing tilapia cultivation in Gelam Jaya Village, and has a value above 0.50.
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