Melinjo seeds are one of the plants that have the potential to be developed as anticancer drugs. This study aimed to determine the cytotoxic activity of melinjo seed protein against MCF-7 and vero cells, and to determine the antiproliferative activity of melinjo seed protein against MCF-7 cells. The method used for the isolation of melinjo seed protein was SDS-PAGE. Cytotoxic and antiproliferative activities were carried out using the MTT assay method and the absorbance was read on an ELISA reader. The results showed that melinjo seed protein had cytotoxic activity against MCF-7 cells with IC 50 of 125.89 μg/mL. The IC 50 results of melinjo seed protein against vero were 3.37 μg/mL. Melinjo seed protein was not selective against vero cells where the SI values were 0.03. In the antiproliferative activity test of melinjo seed protein, the doubling time at levels of 1.875 μg/mL was 86.55 h, while the control cell doubling time was 112.32 h. These results indicated that melinjo seed protein was not able to inhibit the proliferation of MCF-7 cells.
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