A rainfall threshold for landslide occurrence at a national scale in China has rarely been developed in the early warning system for landslides. Based on 771 landslide events that occurred in China during 1998–2017, four groups of rainfall thresholds at different quantile levels of the quantile regression for landslide occurrences in China are defined, which include the original rainfall event–duration (E–D) thresholds and normalized (the accumulated rainfall is normalized by mean annual precipitation) (EMAP–D) rainfall thresholds based on the merged rainfall and the Climate Prediction Center Morphing technique (CMORPH) rainfall products, respectively. Each group consists of four sub-thresholds in rainy season and non-rainy season, and both are divided into short duration (<48 h) and long duration (≥48 h). The results show that the slope of the regression line for the thresholds in the events with long durations is larger than that with short durations. In addition, the rainfall thresholds in the non-rainy season are generally lower than those in the rainy season. The E–D thresholds defined in this paper are generally lower than other thresholds in previous studies on a global scale, and a regional or national scale in China. This might be due to there being more landslide events used in this paper, as well as the combined effects of special geological environment, climate condition and human activities in China. Compared with the previous landslide model, the positive rates of the rainfall thresholds for landslides have increased by 16%–20%, 10%–17% and 20%–38% in the whole year, rainy season and non-rainy season, respectively.

Endoplasmic reticulum stress (ERS) and autophagy activation play important roles in the process of cerebral ischemia/reperfusion (I/R) injury. The synergistic protective effects of Geniposide and ursodeoxycholic acid against cellular apoptosis caused by oxygen-glucose deprivation-reoxygenation (OGD/R) were investigated using a Cell Counting Kit-8 assay, lactate dehydrogenase (LDH) assay, flow cytometry, quantitative polymerase chain reaction (qPCR), and western blotting to examine cellular viability, apoptosis, reactive oxygen species (ROS) levels, mRNA and protein levels, respectively, in relation to ERS and autophagy. We found that pretreatment with Geniposide improved cellular viability. Moreover, treatment with a combination of Geniposide and Tauroursodeoxycholic acid (TUDCA) (GT) protected injured cells better than Geniposide alone. Further studies showed that the increase in cellular ROS levels, and the overexpression of mRNA and proteins related to OGD/R-induced ERS and autophagy, were both counteracted by GT. Our study indicates that the protective effects of GT on OGD/R-induced apoptosis in SH-SY5Y cells are associated with the inhibition of ERS and autophagy.

The method for the determination of imidaclothiz residue in tea by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The imidaclothiz in tea was extracted by acetonitrile and purified by QuEChERS with PSA (primary secondary amine), C18, GCB (graphitized carbon black) as the adsorbents. The purified solution was centrifuged and the supernatant was diluted with water of equal volume. The separation was performed on a C18 column with a gradient elution program of acetonitrile (containing 0.1% (v/v) formic acid) and water at a flow rate of 0.30 mL/min. The mass spectrometer was carried out with electrospray ion source in the positive mode (ESI+) and selective reaction monitoring (SRM), quantified by external standard solution. The results showed that the mass concentration of imidaclothiz in the range of 1 to 500 μg/L was linearly correlated with the peak area, and the correlation coefficient (r) was 0.999 9. The limit of quantification (LOQ, S/N ≥ 10) was 0.01 mg/kg. The recoveries in oolong tea and green tea at three spiked levels (0.01, 0.3 and 3 mg/kg) varied from 87.0%-101.0% and the relative standard deviations (RSDs, n = 7) were between 2.1% and 13.1%. The real sample tests showed that the method is simple, cheap, accurate, specific, rapid, and suitable for the qualitative and quantitative confirmation of imidaclothiz residue in tea.

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