Peony is a famous ornamental and medicinal plant in China, and peony hybrid breeding is an important means of germplasm innovation. However, research on the genome of this species is limited, thereby hindering the genetic and breeding research on peony. In the present study, simple sequence repeat (SSR) locus analysis was performed on expressed sequence tags obtained by the transcriptome sequencing of Paeonia using Microsatellite software. Primers with polymorphism were obtained via polymerase chain reaction amplification and electrophoresis. As a result, a total of 86,195 unigenes were obtained by assembling the transcriptome data of Paeonia. Functional annotations were obtained in seven functional databases including 49,172 (Non-Redundant Protein Sequence Database: 57.998 SSR loci were distributed in 17,567 unigenes containing SSR sequences, and the SSR distribution frequency was 25.52%, with an average of one SSR sequence per 4.66 kb. Mononucleotide, dinucleotide, and trinucleotide were the main repeat types, accounting for 55.74%, 25.58%, and 13.21% of the total repeat times, respectively. Forty-five pairs of the 100 pairs of primers selected randomly could amplify clear polymorphic bands. The polymorphic primers of these 45 pairs were used to cluster and analyze 16 species of peony. The new SSR molecular markers can be useful for the study of genetic diversity and marker-assisted breeding of peony.

This research reports for the first time the epidermal characteristics of 10 wild tree peonies belonging to the sect. Moutan group. The leaf micromorphology of 10 wild tree peonies—P. qiui, P. decomposita, P. jishanensis, P. ostii, P. delavayi, P. lutea, P. potanini, P. rockii, P. ludlowii and P. cathayana—were investigated by light and scanning electron microscopy. The characteristics of the leaf epidermis were found to be constant at the species level, but variable among species. Patterns of anticlinal walls were slightly wavy to wavy, very wavy, and straight to curved. All studied tree peonies presented stomata only on the abaxial surfaces of the leaves. The stomatal apparatus was elliptical or rectangular. Stomatal density was highest on the abaxial surface in P. qiui (300.25 per mm2), and lowest in P. jishanensiss (198.41 per mm2). Stomatal Index was highest on the adaxial surface of P. potanini (27.30%), and lowest in P. ludlowii (18.35%). Trichomes were observed in four studied peonies, which appeared only on the abaxial surface of the leaves, and three basic types of trichomes were identified. The long conical trichomes were distinctive of P. jishanensis, and the flat-shaped trichomes were characteristic of P. qiui, P. rockii and P. cathayana, and commonly occurred in the intervenous region and leaf veins. Stomatal shape, stomatal density, anticlinal wall patterns and trichomes may be of great value and significance for the classification of wild tree peonies. The principal component analysis showed that the component 1, component 2 and component 3 were the most components and nearly 79% of the observed variation. The key to the identification of wild tree peonies based on trichomes and stomata diversity was provided.

Dormancy is important for the pear (Pyrus pyrifolia) to survive a harsh environment. The molecular base of dormancy in pear, especially in some local cultivars, is still unclear. Genome-wide transcriptome analysis in flower buds of cv. Huangli (an excellent local cultivar native to Guizhou mountain area in China) was conducted to explore the mechanism regulating bud dormancy in pear. For the release of endo-dormancy 223 chilling hours (CHs) was needed in Huangli flower buds, which was less than in commercial cultivars. Comparisons of transcript amounts among seven dates during dormancy