The present study was carried out to optimize the different conditions for cross‐linked β‐cyclodextrin (β‐CD) using adipic acid on cholesterol removal in cream. Different factors were β‐CD concentration, mixing temperature, mixing time and mixing speed. Cross‐linked β‐CD was prepared with adipic acid. When the cream was treated with 10% cross‐linked β‐CD, cholesterol removal was the highest at 90.7%, which was not significantly different from treatments with 15% and 20% cross‐linked β‐CD. Cholesterol removal was significantly influenced by mixing temperature, mixing time and mixing speed. After cholesterol removal from cream, the used cross‐linked β‐CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal in first trial was 91.5%, which was mostly same as that using new cross‐linked β‐CD. However, after 10 repeated trials using the same sample, only 83.4% of the cholesterol was removed from cream. Therefore, the present study indicated that the optimum conditions for cholesterol removal using cross‐linked β‐CD were 10%β‐CD addition, 30 min mixing with 1400 r.p.m. speed at 40°C with over 90% cholesterol removal. In addition, recycled β‐CD cross‐linked with adipic acid showed a similarly high recycling efficiency to unused β‐CD of about 90% up to the seventh trial.

The present study was carried out to develop crosslinking of β-cyclodextrin (β-CD) using adipic acid, and to determine the optimum conditions of different factors (β-CD concentration, mixing temperature, mixing time and mixing speed) on cholesterol reduction from homogenized milk. Crosslinked β-CD was prepared with adipic acid. When the milk was treated with different conditions, the cholesterol removal rate was in the range of 92.1 to 93.1% with 1% β-CD addition, which were not significantly different among treatments. After cholesterol removal from milk, the used crosslinked β-CD was washed for cholesterol dissociation and reused. For recycling study, the cholesterol removal rate in the first trial was 92.5%, which was mostly same as that using new crosslinked β-CD. With repeated ten time trials using same sample, 81.4% of cholesterol was removed from milk. Therefore, the present study indicated that the optimum conditions for cholesterol removal using crosslinked β-CD were 1% β-CD addition and 10 min mixing with 400 rpm speed at 5°C with over 90% cholesterol removal. In addition, crosslinked β-CD made by adipic acid resulted in the effective recycling efficiency.