Objective: The objective of this study is to assess the performance of Rapidec carba NP test in the reliable detection of carbapeneme resistance in Gram negative bacteria. Methods: This study was performed at the Microbiology unit, Clinical Pathology Department at Benha University Hospital. A total of 60 stored isolates were included in the study. These isolates were Gram negative bacilli and were characterized phenotypically and genotypically for the presence of carbapenemases. Fifty isolates were confirmed to be carbapenemase + ve and they were used as study group. Ten isolates were confirmed to be carbapenemase -ve and were used as negative control. Rapidec carba NP test was applied according to manufacturer , s instructions and results were read after 30 and 120 minutes of incubation. Results: Ready-to-use Rapidec carba NP gave 100% sensitivity, specificity positive predictive value and negative predictive value when manufacturer ' s instructions were followed. This was in accordance with PCR results. Rapid detection of carbapenemases genes is of great importance, since these MDR organisms have the potential to spread rapidly in hospital environments and cause nosocomial infections with high mortality rates. Molecular methods for detection of carbapenem resistance are the gold standard, however they are expensive, time consuming and require specialized equipment and experience. Conclusion: Rapidec carba NP test is rapid, easy to perform and interpret, relatively inexpensive and present a practical solution for rapid detection of carbapenem resistant gram negative bacteria.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.