Minerals are required by the human body to perform physiological functions. Mineral deficiencies, often caused by low mineral bioaccessibility in plant-based foods, are a matter of great concern all over the world. Several antinutrients (e.g. pectin) may contribute to this reduced mineral bioaccessibility by formation of indigestible complexes due to mineral binding. Structural characteristics of the antinutrients, as for instance the degree of methylesterification (DM) in the case of pectin, may play a role in this mineral binding phenomenon and has been evaluated before, however, only in single mineral model systems. In natural food systems, several mineral types are present together which may affect each other's bioaccessibility. Therefore, this study investigated the influence of the presence of Ca 2+ on Zn 2+ binding capacity and bioaccessibility in mineral-pectin model systems with different DM. The results showed that increasing Ca 2+ concentration and pectin DM reduces the Zn 2+ binding capacity of pectin and consequently increases Zn 2+ bioaccessibility in the in vitro small intestine. Moreover, the in vitro digestion procedure with adjustment of pH only, no addition of enzymes, bile salts nor digestive fluids during simulation of gastric and small intestinal phases, was found to be most appropriate to fundamentally study the influence of pectin DM and presence of Ca 2+ on Zn 2+ bioaccessibility in mineral-pectin model systems. Keywords Citrus pectin, degree of methylesterification, mineral (Ca 2+ and Zn 2+) competition, Zn 2+ binding capacity, Zn 2+ bioaccessibility. number of macromolecules and for more than 300 enzymatic reactions (Gharibzahedi & Jafari, 2017; Jackson & Lowe, 1992). Low zinc blood levels may lead to numerous clinical symptoms, such as growth retardation, impaired brain development and cognitive performance, poor wound healing, diarrhoea, infertility or increased risk of infections (Wapnir, 2000). One of the major contributing factors towards mineral deficiencies is inadequate intake, as minerals cannot be synthesized by the human body and therefore must be obtained from the diet. Inadequate mineral intake can be attributed to low amounts in the ingested food or low mineral bioaccessibility (BAC) from the consumed food products (Platel & Srinivasan, 2015). The term bioaccessibility is defined as the fraction of a nutrient which is released from the food matrix into the gastrointestinal tract through the digestion process and that becomes available for intestinal absorption (Carbonell-Capella, Buniowska, Barba, Esteve, & Frígola, 2014). In plant-based foods, mineral BAC can be reduced due to the presence of several mineral antinutrients (Platel & Srinivasan, 2015), including dietary fibers, phytic acid or polyphenols. These mineral antinutrients can bind in situ minerals, minerals which are added to the food or minerals originating from other simultaneously ingested food ingredients, thereby potentially reducing the mineral release for intestinal absorption (Kumar, Sinha, Makkar, & Beck...