A series of sulfanilamide Schiff base derivatives (1 to 15) have been designed as potential antitubulin agents depending on the chemical structures of combretastatine A-4 and isoquinoline sulfamate (antimitotic agents under investigation). The designed compounds were synthesized by microwave chemical synthesis, their purity was confirmed by melting point and HPLC and chemical structures were determined by FT-IR, UV, and 1H and 13C-NMR spectroscopic techniques. The synthesized compounds have been docked in the colchicine binding site of β-tubulin using molecular modeling programs and the antitumor activities were screened on human breast and lung cancer cells by cell counting assay. Some tested compounds showed potent and selective activity against breast cancer (MCF-7) with IC50 range of 90 to 166 μM. With regarding broad-spectrum activity, compounds 4, 8, and 13 have shown potent antitumor activity against human breast and human lung cells with IC50 range of 96 to 140 μM. The obtained results suggest that the sulfanilamide Schiff base derivatives might potentially constitute an interesting novel class of anticancer agents, which deserve further studies.

Objective: The appearance of resistant bacteria reduces the efficiency of antimicrobial therapies, thereby increasing the need for more efficient drugs for infections treatment. Many studies have shown an enhance in antimicrobial activity after the interaction of many agents with metal ions. Complexes of the metal ions with ligands which are polydentate have been the theme of demanding research as they have interesting spectral, magnetic properties and a miscellaneous spectrum of biological activities.Methods: New isoniazid based compounds and their transition metal complexes (cobalt (II), copper (II), nickel (II) and zinc (II)) were produced using microwave synthesis technique. The All compounds which were synthesized (free ligand and their metal complexes) were fully characterized by many spectroscopic techniques (FT-IR spectra, UV/visible electronic spectra, mass spectra and 13 C NMR and 1 H NMR spectra). In addition, CHN, XRFA, AAS merged with other spectroscopic data were utilized to allocate the precise ligand to metal ratio and geometry. The synthesized ligands and their complexes were tested for in vitro antimicrobial activity against Candida albicans (ATCC 10231), Aspergillus niger (ATCC 16404), Escherichia coli (ATCC 25922), and Staphylococcus aureus (ATCC 29213) by using agar-well diffusion.

The genetic diversity among a group of 20 sorghum accessionsfrom Sudan were assessed with RAPD molecular marker. Out of 572amplified PCR bands scored, 61.1% were polymorphic. Estimates of thegenetic similarity among the accessions ranged from 60% to 93%. Adendrogram, constructed on the basis of similarly matrix data, categorizedthe accessions into two main groups: a major one comprising nine groupsinvolving 14 accessions and a smaller one consisting of two groupsinvolving four accessions. The race Caudatum and its hybrids and theDurra race and its hybrids were detected in 18 and 16 of the accessionstested, respectively, whereas the races Bicolor and Guinea wererepresented each by only one accession and Kafir by two accessions.There was agreement between the relationships revealed by molecularcharacterization and those reflected by racial classification in some casesbut not in others. Thus, in future, to ensure a more complete andinformative characterization of the germplasm., both molecular andmorphological methods should be used.